Multiple regulatory elements of the murine gamma 2-crystallin promoter

Abstract

Crystallins are the major water-soluble proteins of the vertebrate eye lens. These lens-specific proteins are encoded by several multi-gene families whose expression is differentially regulated during development. Our previous studies showed that the mouse gamma 2-crystallin promoter is active on transfection into lens-explant cultures derived from 14-day-old chick embryos but not on transfection into a variety of non-lens cells. In this study, transient expression data show that a sequence of 226 nucleotides upstream from the transcription start site is sufficient for activity of this promoter in the chicken lens cells. This sequence can be further divided into two domains, A and B, both of which are required for promoter function. Domain A (nucleotide -68 to -18) contains the TATA box and sequence motifs that are conserved in all gamma-crystallin promoters. Domain B (-226 to -120) consists of three regions. One of these regions contains an element with dyad symmetry and a sequence similar to the octamer motif. The second region contains an enhancer core consensus sequence. Two "enhancer-like" activities have been detected, one in Domain B and a second in a more distal region (-392 to -278) that does not appear to be required for promoter activity in transfection assays.