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. 1989 May;86(10):3474-8.
doi: 10.1073/pnas.86.10.3474.

Characterization of oligonucleotide transport into living cells

S L Loke  1 , C A Stein, X H Zhang, K Mori, M Nakanishi, C Subasinghe, J S Cohen, L M Neckers
Affiliations

Characterization of oligonucleotide transport into living cells

S L Loke et al. Proc Natl Acad Sci U S A. 1989 May.

Abstract

Addition of antisense oligonucleotides to cell cultures has been used to specifically inhibit gene expression. We have investigated the mechanism by which oligonucleotides enter living cells. These compounds are taken up by cells in a saturable, size-dependent manner compatible with receptor-mediated endocytosis. Polynucleotides of any length are competitive inhibitors of oligomer transport, providing they possess a 5'-phosphate moiety. Using oligo(dT)-cellulose for affinity purification, we have identified an 80-kDa surface protein that may mediate transport. Knowledge of the oligonucleotide transport mechanism should facilitate the design of more effective synthetic antisense oligomers as potential clinical agents.

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References

    1. Annu Rev Biochem. 1977;46:669-722 - PubMed
    1. Gene. 1988 Dec 10;72(1-2):333-41 - PubMed
    1. Eur J Biochem. 1978 Jul 3;87(3):459-65 - PubMed
    1. Cell. 1980 Aug;21(1):67-77 - PubMed
    1. Biochemistry. 1981 Mar 31;20(7):1874-80 - PubMed

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