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. 2016 Jun 1;12(3):529-37.
doi: 10.5114/aoms.2016.59926. Epub 2016 May 18.

Platelet microparticle number is associated with the extent of myocardial damage in acute myocardial infarction

Anggoro Budi Hartopo  1 , Ira Puspitawati  2 , Putrika Prastuti Ratna Gharini  1 , Budi Yuli Setianto  1
Affiliations

Platelet microparticle number is associated with the extent of myocardial damage in acute myocardial infarction

Anggoro Budi Hartopo et al. Arch Med Sci. .

Abstract

Introduction: Activated platelets generate microparticles. Increased platelet microparticles occur in acute myocardial infarction (AMI) and contribute to intracoronary thrombosis and subsequent myocardial injury. This study aimed to investigate the impact of platelet microparticles on intracoronary thrombosis by assessing the relationship between platelet microparticles and the extent of myocardial damage in AMI.

Material and methods: This was a cross sectional study. The subjects were patients with acute coronary syndrome (ACS). Forty-one consecutive subjects with ACS admitted to intensive cardiovascular care unit were enrolled. The clinical spectrum of ACS comprised AMI (n = 26), both ST-elevation AMI (STEMI) and non-ST-elevation AMI (NSTEMI), and unstable angina (n = 15). Platelet microparticles were isolated from peripheral venous blood and detected with anti-CD42b-PE by the flow cytometry method. The extent of myocardial damage was determined by measuring the peak level of serial cardiac enzymes within 24 h of admission.

Results: Subjects with AMI had a significantly higher number of platelet microparticles than those with unstable angina (4855 ±4509/µl vs. 2181 ±1923/µl respectively; p = 0.036). Subjects with STEMI had the highest number of platelet microparticles, but no significant difference was detected as compared to those with NSTEMI (5775 ±5680/µl vs. 3601 ±1632/µl). The number of platelet microparticles in AMI was positively associated with the extent of myocardial damage (peak CK-MB: r = 0.408, p = 0.019 and peak GOT: r = 0.384, p = 0.026).

Conclusions: The number of platelet microparticles was increased in AMI as compared to unstable angina and associated with the extent of myocardial damage.

Keywords: acute myocardial infarction; myocardial damage; platelet microparticle; thrombosis.

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Figures

Figure 1
Figure 1
The number of platelet microparticles was significantly higher in subjects with AMI as compared to those with unstable angina (Student's t-test: p = 0.036)
Figure 2
Figure 2
The number of platelet microparticles according to clinical spectrums of ACS. STEMI had the highest number of platelet microparticles (ANOVA test: p = 0.040), but it did not differ significantly with NSTEMI (post-hoc LSD: p = 0.178), whereas it differed significantly with unstable angina (posthoc LSD: p = 0.012), which had the lowest amount of platelet microparticles
Figure 3
Figure 3
Representative dot-plot of CD42b positivity of platelet microparticles from flow cytometry analysis according to clinical spectrums of ACS. R2 was gated for CD42b+ and the threshold of 1.5 μm, indicating platelet microparticle. R1 was gated for collected TruCount bead events
Figure 4
Figure 4
Positive and significant correlation between an increased number of platelet microparticles and a peak level of GOT and CK-MB, indicating the extent of myocardial damage, in subjects with AMI
See this image and copyright information in PMC

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