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. 2016 May 25;2(5):274-7.
doi: 10.1021/acscentsci.6b00110. Epub 2016 May 2.

Mysteries in a Minimal Genome

Maxwell Coyle  1 , Jennifer Hu  1 , Zev Gartner  1
Affiliations

Mysteries in a Minimal Genome

Maxwell Coyle et al. ACS Cent Sci. .
No abstract available

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Figures

Figure 1
Figure 1
Beginning with solid-phase DNA synthesis from phosphoramidite precursors, and continuing with a series of PCR, in vitro assembly, and cloning steps, Venter and colleagues developed a workflow for synthesizing synthetic cellular genomes in ∼3 weeks. Breaking the genome into one-eighth pieces allowed them to minimize each segment independently and test it against a seven-eights wild-type background. Artwork credit: Jennifer Hu.
Figure 2
Figure 2
Once the genome of JCVI-syn3.0 was “booted up” in the cytoplasm of wild-type M. capricolum, the cells were able to divide themselves, with a doubling time of approximately 3 h. Reproduced with permission from ref (8). Copyright 2016 American Association for the Advancement of Science.
See this image and copyright information in PMC

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