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. 2016 Aug;54(8):2197-200.
doi: 10.1128/JCM.01156-16. Epub 2016 Jun 8.

Single-Step Multiplex PCR Assay for Determining 92 Pneumococcal Serotypes

José M Marimón  1 , María Ercibengoa  2 , Erica Santacatterina  3 , Marta Alonso  4 , Emilio Pérez-Trallero  5
Affiliations

Single-Step Multiplex PCR Assay for Determining 92 Pneumococcal Serotypes

José M Marimón et al. J Clin Microbiol. 2016 Aug.

Abstract

For pneumococcal disease surveillance, simple and cost-effective methods capable of determining all serotypes are needed. Combining a single-tube multiplex PCR with fluorescently labeled primers followed by amplicon analysis using automated fluorescent capillary electrophoresis, each serotype of 92 reference isolates and 297 recently collected clinical isolates was successfully determined.

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