Association of TNFα-308, IFNγ+874, and IL10-1082 gene polymorphisms and the risk of non-small cell lung cancer in the population of the South Indian state of Telangana

Abstract

Background: Cytokine-mediated inflammation is important in the pathogenesis of non-small cell lung cancer (NSCLC). Genetic polymorphisms in cytokine genes and their association with lung cancer in the Indian population have not been reported.

Methods: For the first time, we analyzed genetic polymorphisms of TNFα ^-308, IFNγ ⁺⁸⁷⁴, and IL10 ^-1082 genes in 246 NSCLC patients and 250 healthy controls in the South Indian population from Telangana using ARMS PCR.

Results: IFNγ⁺⁸⁷⁴ A/T and IL10^-1082 G/G gene polymorphisms were found to be significantly associated with NSCLC with 1.56- and 1.68-fold disease risk, respectively. There was no association between the risk of NSCLC and TNFα^-308 polymorphism. Gene polymorphisms stratified according to smoking revealed that IFNγ⁺⁸⁷⁴ A/T polymorphisms in smokers increased the disease risk by 2.91 fold. IL10^-1082 G/G polymorphisms showed 2-fold increased risk among patients who were smokers when compared to the controls. However, there was no association between TNFα^-308, IFNγ⁺⁸⁷⁴, and IL10^-1082 gene polymorphism and the stage of the NSCLC patients. The overall risk associated with the combination of these polymorphisms indicated that the TNFα^-308 G/A + IFNγ⁺⁸⁷⁴ A/T + IL10^-1082 G/G genotype increased the risk by 1.5 fold.

Conclusions: The results of our study indicate an association between cytokine gene polymorphisms and the risk of NSCLC in an Indian population.

Keywords: Gene polymorphism; Interferon-γ; Interleukin-10; Non-small cell lung cancer; Tumor necrosis factor-α.