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. 2016 Feb 11;55(2):121-5.
doi: 10.1515/sjph-2016-0018. eCollection 2016 Jun 1.

Infections Caused By Community-Associated Methicillin-Resistant Staphylococcus Aureus European Clone (ST80) In Slovenia Between 2006 And 2013: PRIKAZ PRIMEROV OKUŽB, POVZROČENIH S PROTI METICILINU ODPORNO BAKTERIJO STAPHYLOCOCCUS AUREUS, DOMAČEGA OKOLJA, KI PRIPADA EVROPSKEMU KLONU (ST80) V SLOVENIJI V OBDOBJU MED 2006 IN 2013

Urška Dermota  1 , Tomaž Jurca  2 , Tatjana Harlander  3 , Marta Košir  4 , Urška Zajc  5 , Majda Golob  5 , Irena Zdovc  5 , Irena Grmek Košnik  1
Affiliations

Infections Caused By Community-Associated Methicillin-Resistant Staphylococcus Aureus European Clone (ST80) In Slovenia Between 2006 And 2013: PRIKAZ PRIMEROV OKUŽB, POVZROČENIH S PROTI METICILINU ODPORNO BAKTERIJO STAPHYLOCOCCUS AUREUS, DOMAČEGA OKOLJA, KI PRIPADA EVROPSKEMU KLONU (ST80) V SLOVENIJI V OBDOBJU MED 2006 IN 2013

Urška Dermota et al. Zdr Varst. .

Abstract
in English, Slovenian

Introduction: According to the existing literature, a heterogeneous sequence type (ST) or clones of community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) circulate in Europe. In Europe, the European clone that belongs to sequence type ST80 is predominant.

Methods: The aim of the study was to investigate the phenotypic and genotypic characteristics and epidemiological data of CA-MRSA ST80 and its occurrence in Slovenia. We retrospectively analyzed those CA-MRSA isolates that were isolated during microbiological procedures in microbiological laboratories between 2006 and 2013. Only CA-MRSA isolates from the national collection of CA-MRSA strains that belonged to ST80 (European clone) were analyzed. We determined the Pantone-Valentine leukocidin (PVL), mec A genes, exfoliative toxin genes and type of staphylococcal cassette chromosome (SCCmec) by polymerase chain reaction (PCR). We determined also spa type and sequence type.

Results: ST80 was confirmed in only 2 (0.5%) out of 385 CA-MRSA isolates, collected in a national collection of CAMRSA. Both isolates were positive for the PVL genes, mec A gene, exfoliative toxin type D gene and SCCmec IV. One CA-MRSA isolate was confirmed in a wound swab taken from a 47-year-old male, and the second was isolated from blood cultures of a 69-year-old female. No epidemiological connections between them were found.

Conclusions: In Slovenia CA-MRSA infections caused by ST80 are rare. In the future, it is necessary that a surveillance study of CA-MRSA at the national level continues and CA-MRSA be considered as a public health threat.

Izhodišča: Po podatkih iz literature kroži v Evropi zelo heterogena skupina sekvenčnih tipov (ST) in klonov proti meticilinu odporne bakterije Staphylococcus aureus, domačega okolja (CA-MRSA). V Evropi med CA-MRSA prevladuje evropski klon, ki pripada sekvenčnemu tipu ST80.

Metode: V raziskavi smo želeli pridobiti informacijo o fenotipskih in genotipskih lastnostih ter epidemiološke podatke o CA-MRSA, ki pripadajo sekvenčnemu tipu ST80 in njihovo razširjenost v Sloveniji. Retrospektivno smo pregledali izolate CA-MRSA, ki so bili osamljeni med rutinsko mikrobiološko diagnostiko v mikrobioloških laboratorijih v obdobju od 2006 do 2013. Analizirali smo le izolate CA-MRSA, ki so bili vključeni v nacionalno zbirko izolatov CAMRSA in so pripadali sekvenčnemu tipu ST80 (evropski klon). Za lažjo opredelitev CA-MRSA ST80 smo uporabili tudi verižno reakcijo s polimerazo (PCR), s katero smo določili tip kasete stafilokoknega kromosoma (SCCmec), gene mecA, levkocidina Pantone-Valentine (PVL) in tip stafilokoknega eksfoliativnega toksina. Izolatom CA-MRSA smo določili tudi tip spa in sekvenčni tip.

Rezultati: ST80 smo potrdili pri dveh (0,5%) od 385 izolatov CA-MRSA, zbranih v nacionalni zbirki CA-MRSA. Pri obeh izolatih smo dokazali gene PVL, mecA, eksfoliativni toksin tipa D in tip SCCmec IV. En izolat CA-MRSA smo dokazali iz brisa rane 47-letnega bolnika, drug izolat pa pri 69-letni bolnici iz hemokulture. Oba primera nista bila epidemiološko povezana.

Zaključki: Okužbe, povzročene s CA-MRSA ST80 v Sloveniji, so redke. V prihodnosti je pomembno, da s sledenjem CA-MRSA na nacionalnem nivoju nadaljujemo, saj CA-MRSA po svetu predstavljajo novo grožnjo javnemu zdravju prebivalcev.

Keywords: CA-MRSA; ST80 (European clone); ST80 (evropski klon); Slovenia; Slovenija; death; invasive infections; invazivne okužbe; smrt.

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Conflict of interest statement

The authors declare that no conflicts of interest exist.

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