Immunohistochemistry and scoring of Ki-67 proliferative index and p53 expression in gastric B cell lymphoma from Northern African population: a pilot study

Abstract

Background: This study aimed to clarify the Ki-67 distribution, p53 expression and their relationship with clinico-pathologic features of gastric B cell lymphoma from Northern African population.

Methods: Twenty paraffin blocks of gastric lymphoma were retrieved from the archival materials of Department of Pathology, Central University Hospital of Sidi Bel Abbes (Western Algeria) from 2007 to 2013. Four µm section specimens were stained by immunohistochemical (IHC) technique with Ki-67 and p53 tumor markers. P values <0.05 were considered statistically significant.

Results: Expression of p53 proteins and the mean proliferative index (PI) were compared between high grade gastric B cell lymphomas (DLBCL) and low grade gastric B cell lymphomas (gastric MALTs). p53 overexpression (P=0.007) and a high proliferation index Ki-67 (P=0.001) were significantly associated with gastric DLBCL. We found also a statistically significant correlation between p53 and Ki-67 (P=0.007) but no obvious relationships were found between Ki-67 PI and p53 expression as well as clinico-pathological features (age, sex, location, macroscopic type).

Conclusions: The IHC studies of Ki-67 and p53 expression in gastric B cell lymphoma can help in monitoring of patients at risk, and to give suitable treatment and management of patients.

Keywords: Ki-67; Northern African population; gastric B cell lymphoma; immunohistochemistry; p53.

Figure 1

Frequency of gastric B cell lymphoma.

Figure 2

Expressions of Ki-67 and p53 in gastric diffuse large B cell lymphoma. (A) Diffuse large B cell lymphoma in stomach biopsy (H&E, 200×); (B) Ki-67 shows a high proliferative index in gastric DLBCL, (Ki-67 immunohistochemistry, 200×); (C) p53 protein overexpression is noted in the tumor cells of DBLCL (p53 immunohistochemistry, 200×). H&E, haematoxyline and eosin.