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. 2016 Jun 23;23(6):709-15.
doi: 10.1016/j.chembiol.2016.05.009. Epub 2016 Jun 9.

Ancestral Tryptophan Synthase Reveals Functional Sophistication of Primordial Enzyme Complexes

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Ancestral Tryptophan Synthase Reveals Functional Sophistication of Primordial Enzyme Complexes

Florian Busch et al. Cell Chem Biol. .
Free article

Abstract

Modern enzyme complexes are characterized by a high catalytic efficiency and allosteric communication between the constituting protein subunits. We were interested in whether primordial enzyme complexes from extinct species displayed a similar degree of functional sophistication. To this end, we used ancestral sequence reconstruction to resurrect the α and β subunits of the tryptophan synthase (TS) complex from the last bacterial common ancestor (LBCA), which presumably existed more than 3.4 billion years ago. We show that the LBCA TS subunits are thermostable and exhibit high catalytic activity. Moreover, they form a complex with αββα stoichiometry whose crystal structure is similar to that of modern TS. Kinetic analysis revealed that the reaction intermediate indole is channeled from the α to the β subunits and suggests that allosteric communication already occurred in LBCA TS.

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