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. 2016 May 21:8:114-9.
doi: 10.1016/j.dib.2016.05.031. eCollection 2016 Sep.

Peptide isolated from Cry1Ab16 toxin present in Bacillus thuringiensis: Synthesis and morphology data for layer-by-layer films studied by atomic force microscopy

Alexandra Plácido  1 , Emanuel Airton de Oliveira Farias  2 , Mariela M Marani  3 , Andreanne Gomes Vasconcelos  2 , José R S A Leite  2 , Cristina Delerue-Matos  1
Affiliations

Peptide isolated from Cry1Ab16 toxin present in Bacillus thuringiensis: Synthesis and morphology data for layer-by-layer films studied by atomic force microscopy

Alexandra Plácido et al. Data Brief. .

Abstract

The peptide PcL342-354C was obtained from the Cry1Ab16 toxin present in Bacillus thuringiensis ("Computational Modeling Deduced Three Dimensional Structure of Cry1Ab16 Toxin from B. thuringiensis AC11" (Kashyap, 2012) [1]). In this data article, we report the synthesis and characterization of the PcL342-354C peptide by MALDI-TOF/TOF mass spectrometry. In addition, the preparation of layer-by-layer films is shown based on interspersion of this peptide with both polyethylenimine (PEI) and poly(sodium 4-styrenesulfonate) (PSS), self-assembled on ITO (indium tin oxide) electrodes. The morphology of the ITO/PEI/PSS/PcL342-354C film was analyzed using atomic force microscopy (AFM). We also evaluated the effect of the number of bilayers in ITO/PEI/(PSS/PcL342-354C) n on the morphology of the film using AFM amplitude images. Further details about this study were published elsewhere, "Layer-by-layer films containing peptides of the Cry1Ab16 toxin from B. thuringiensis for potential biotechnological applications," (Plácido et al., 2016) [2].

Keywords: Atomic force microscopy; Bacillus thuringiensis; Cry1Ab16 toxin; GMO׳s; Layer-by-layer films.

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Figures

Fig. 1.
Fig. 1
Purification of the PcL342-354C peptide in the analytical RP-HPLC system.
Fig. 2.
Fig. 2
MS analysis of the PcL342-354C peptide ([M+H]+=1469.71 Da).
Fig. 3.
Fig. 3
Analysis of the MS/MS spectrum of the PcL342-354C peptide.
Fig. 4.
Fig. 4
AFM amplitude images for glass surfaces covered with: (A) ITO, LbL films of (B) ITO/PEI, (C) ITO/PEI/PSS, (D) ITO/PEI/PSS/PcL342-354C, (E) ITO/PEI/(PSS/PcL342-354C)2, and (F) ITO/PEI/(PSS/PcL342-354C)5. All images are 4×4 µm in x and y.
See this image and copyright information in PMC

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