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. 2016 Jun 14;11(6):e0156835.
doi: 10.1371/journal.pone.0156835. eCollection 2016.

Influence of Oleic Acid on Rumen Fermentation and Fatty Acid Formation In Vitro

Duanqin Wu  1   2 , Liwei Xu  1 , Shaoxun Tang  1 , Leluo Guan  3 , Zhixiong He  1 , Yongjuan Guan  4 , Zhiliang Tan  1 , Xuefeng Han  1 , Chuanshe Zhou  1 , Jinhe Kang  1 , Min Wang  1
Affiliations

Influence of Oleic Acid on Rumen Fermentation and Fatty Acid Formation In Vitro

Duanqin Wu et al. PLoS One. .

Abstract

A series of batch cultures were conducted to investigate the effects of oleic acid (OA) on in vitro ruminal dry matter degradability (IVDMD), gas production, methane (CH4) and hydrogen (H2) production, and proportion of fatty acids. Rumen fluid was collected from fistulated goats, diluted with incubation buffer, and then incubated with 500 mg Leymus chinensis meal supplemented with different amounts of OA (0, 20, 40, and 60 mg for the CON, OA20, OA40 and OA60 groups, respectively). Incubation was carried out anaerobically at 39°C for 48 h, and the samples were taken at 12, 24 and 48 h and subjected to laboratory analysis. Supplementation of OA decreased IVDMD, the cumulative gas production, theoretical maximum of gas production and CH4 production, but increased H2 production. However, no effect was observed on any parameters of rumen fermentation (pH, ammonia, production of acetate, propionate and butyrate and total volatile fatty acid production). The concentrations of some beneficial fatty acids, such as cis monounsaturated fatty acids and conjugated linoleic acid (CLA) were higher (P < 0.05) from OA groups than those from the control group at 12 h incubation. In summary, these results suggest that the OA supplementation in diet can reduce methane production and increase the amount of some beneficial fatty acids in vitro.

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Conflict of interest statement

Competing Interests: The authors have declared that no competing interests exist.

Figures

Fig 1
Fig 1. The in vitro dry matter degradability of Leymus chinensis in response to different levels of OA supplementation.
All values are mean ± SEM. a, b, c, d Means that are sharing different superscripts are different (P < 0.05) within the same incubation stage.
Fig 2
Fig 2. In vitro accumulative gas production of Leymus chinensis supplemented with OA.
Fig 3
Fig 3. Effects of OA supplementation on CH4 (I) and H2 production (II) of in vitro incubation.
All values are mean ± SEM. a, b, c Means that are sharing different superscripts are different (P < 0.05) within the same incubation stage.
Fig 4
Fig 4. Effects of OA supplementation on pH (I) and NH3-N concentration (II) of fermentation liquor in vitro.
All values are mean ± SEM. a, b, c Means that are sharing different superscripts are different (P < 0.05) within the same incubation stage.
Fig 5
Fig 5. Effects of OA supplementation on VFAs concentrations of fermentation liquor in vitro.
All values are mean ± SEM. a, b, c Means that are sharing different superscripts are different (P < 0.05) within the same incubation stage.
See this image and copyright information in PMC

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