Vagal afferents inhibit the antidiuresis and antinatriuresis secondary to bilateral carotid occlusion in the chloralose-anesthetized dog

Abstract

It has been reported that bilateral carotid occlusion (BCO) does not alter renal excretory function in conscious dogs on a high-salt diet with intact vagi provided renal perfusion pressure (RPP) is held constant. In contrast, low carotid sinus pressures in chloralose-anesthetized dogs with severed vagi elicit significant reductions in renal excretory function which were mediated by renal sympathetic nerves. The purpose of the present study was to investigate the influence of BCO on renal function in chloralose-anesthetized, volume expanded dogs with and without intact cervical vagi and with RPP held constant. A total of 10 dogs, volume expanded with hypotonic saline, were prepared to measure systemic arterial pressure (SAP), carotid sinus pressure (CSP), RPP, and urine flow. With the cervical vagi intact, BCO elicited an increase in SAP from 132 +/- 4 mm Hg to 172 +/- 5 mm Hg (p less than 0.01). This was associated with significant and paradoxical increases in urine flow (+35%), sodium excretion (+63%), osmolar clearance (+32%), and free water clearance (+33%). These changes were also accompanied by small but significant increases in ERBF (+11%) and GFR (+12%). Bilateral cervical vagotomy alone (Vx) increased SAP (129 +/- 6 mm Hg to 146 +/- 5 mm Hg, p less than 0.05). Urine flow and free water clearance were decreased but sodium excretion and osmolar clearance were increased. No change in ERBF or GFR was measured. BCO after Vx elicited a greater increase in SAP as compared to BCO alone (194.5 +/- 6.2 mm Hg) which was accompanied by significant decreases in urine flow (-60%), sodium excretion (-55%), osmolar clearance (-43%), ERBF (-13%), and GFR (-19%). Therefore, the results of this study demonstrate that the contrasting influences of BCO on renal function reported previously in conscious and anesthetized animal models may be due to the presence or absence of the inhibitory influences of afferent fibers contained in the cervical vagi, probably cardiac in origin.