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. 2016 Jan 6;10(3):381-91.
eCollection 2016 Sep.

Molecular Assay on Crimean Congo Hemorrhagic Fever Virus in Ticks (Ixodidae) Collected from Kermanshah Province, Western Iran

Maria Mohammadian  1 , Sadegh Chinikar  2 , Zakkyeh Telmadarraiy  1 , Hassan Vatandoost  1 , Mohammad Ali Oshaghi  1 , Ahmad Ali Hanafi-Bojd  1 , Mohammad Mehdi Sedaghat  1 , Mehdi Noroozi  3 , Faezeh Faghihi  4 , Tahmineh Jalali  2 , Sahar Khakifirouz  2 , Nariman Shahhosseini  5 , Firoozeh Farhadpour  1
Affiliations

Molecular Assay on Crimean Congo Hemorrhagic Fever Virus in Ticks (Ixodidae) Collected from Kermanshah Province, Western Iran

Maria Mohammadian et al. J Arthropod Borne Dis. .

Abstract

Background: Crimean-Congo Hemorrhagic Fever (CCHF) is a feverous and hemorrhagic disease endemic in some parts of Iran and caused by an arbovirus related to Bunyaviridae family and Nairovirusgenus. The main virus reservoir in the nature is ticks, however small vertebrates and a wide range of domestic and wild animals are regarded as reservoir hosts. This study was conducted to determine the infection rate of CCHF virus in hard ticks of Sarpole-Zahab County, Kermanshah province, west of Iran.

Methods: From total number of 851 collected ticks from 8 villages, 131 ticks were selected randomlyand investigated for detection of CCHF virus using RT-PCR.

Results: The virus was found in 3.8% of the tested ticks. Hyalommaanatolicum, H. asiaticum and Rhipicephalus sanguineus species were found to have viral infection, with the highest infection rate (11.11%) in Rh. sanguineus.

Conclusion: These findings provide epidemiological evidence for planning control strategies of the disease in the study area.

Keywords: CCHFV; Iran; Ixodidae; Kermanshah.

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Figures

Fig. 1.
Fig. 1.
Map of the Study area in Kermanshah Province, Iran
Fig. 2.
Fig. 2.
RT-PCR products of CCHF S-segment (536 bp band) found in tick specimens collected in Sarpole-Zahab County, Kermanshah Province. Lad: 100 bp ladder, PC: positive control, NC: negative control, S1, S2, S3, S4, S5, S6, S7 and S9: negative samples; S8 and S10: positive samples.
Fig. 3.
Fig. 3.
Details of CCHF infected ticks and their animal hosts in Sarpole-Zahab County, Kermanshah, Western Iran
Fig. 4.
Fig. 4.
Maximum Likelihood phylogenetic tree retrieved from 500bp of CCHFV partial S-segment sequences obtained in this study (CCHFM14, CCHFM16, CCHFM18, CCHFM22 and CCHFM24) and the available data from Genbank. Only boot strap values more than 70% are shown.
See this image and copyright information in PMC

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