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. 2016 May 30:9:3213-21.
doi: 10.2147/OTT.S98322. eCollection 2016.

The evaluative value of Sema3C and MFN2 co-expression detected by immunohistochemistry for prognosis in hepatocellular carcinoma patients after hepatectomy

Xu Feng  1 , Kelei Zhu  2 , Jinghua Liu  1 , Jiang Chen  1 , Jiacheng Tang  3 , Yuelong Liang  1 , Renan Jin  1 , Xiao Liang  1 , Xiujun Cai  4
Affiliations

The evaluative value of Sema3C and MFN2 co-expression detected by immunohistochemistry for prognosis in hepatocellular carcinoma patients after hepatectomy

Xu Feng et al. Onco Targets Ther. .

Abstract

Background: The ability to evaluate the prognosis of hepatocellular carcinoma (HCC) following hepatectomy using biological markers is of great importance.

Materials and methods: In this study, we collected samples from 54 patients with HCC after hepatectomy. Immunohistochemistry was used to detect the expression of Sema3C and MFN2 in the HCC samples.

Results: Immunohistochemistry results showed that Sema3C and MFN2 co-expression was significantly associated with tumor size. In addition, a significant association between high Sema3C and low MFN2 levels and shorter overall survival was noted, when Sema3C and MFN2 co-expression was analyzed.

Conclusion: The results suggest that the correlative expression level of Sema3C and MFN2 has a strong value in the prognosis of patients with HCC following hepatectomy.

Keywords: MFN2; Sema3C; hepatocellular carcinoma (HCC); prognosis; survival analysis.

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Figures

Figure 1
Figure 1
The expression of Sema3C and MFN2 in HCC and peritumoral tissues. Notes: Positive expression of Sema3C (A) and MFN2 (B) in HCC tissues. Negative expression of Sema3C (C) and MFN2 (D) in peritumoral tissues. Magnification: ×40. Abbreviation: HCC, hepatocellular carcinoma.
Figure 2
Figure 2
IHC showing the correlated expression levels of Sema3C and MFN2 in HCC and PT. Notes: In HCC: (A) Sema3C+/MFN2− (n=24); (B) Sema3C−/MFN2+ (n=15). Magnification: ×10. Abbreviations: HCC, hepatocellular carcinoma; IHC, immunohistochemistry; PT, peritumoral tissues.
Figure 3
Figure 3
Kaplan–Meier curves showing that the expression levels of Sema3C (A) and MFN2 (B) are correlated with prognosis.
Figure 4
Figure 4
Correlation between Sema3C and MFN2 expression levels and survival analysis. Notes: (A) Multivariate analysis of the 54 patients showing a strong anticorrelation between Sema3C and MFN2 expression levels. (B) Kaplan–Meier curves showing the correlation of groups (A–D). Group A: Sema3C+/MFN2−; group B: Sema3C−/MFN2−; group C: Sema3C+/MFN2+; group D: Sema3C−/MFN2+. Abbreviation: HCC, hepatocellular carcinoma.
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