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. 2016:2016:5789381.
doi: 10.1155/2016/5789381. Epub 2016 May 26.

Hematopoietic Effects of Paeoniflorin and Albiflorin on Radiotherapy-Induced Myelosuppression Mice

Yingli Zhu  1 , Linyuan Wang  1 , Zhihui Yang  2 , Jingxia Wang  1 , Wei Li  1 , Jianyu Zhou  1 , Jianjun Zhang  1
Affiliations

Hematopoietic Effects of Paeoniflorin and Albiflorin on Radiotherapy-Induced Myelosuppression Mice

Yingli Zhu et al. Evid Based Complement Alternat Med. 2016.

Abstract

Paeonia lactiflora root (baishao in Chinese) is a commonly used herb in traditional Chinese medicine (TCM). Paeoniflorin (PF) and albiflorin (AF) are two major active constituents of P. lactiflora. In this paper, we aimed to investigate the hematopoietic effects of PF and AF on myelosuppression mice induced by radiotherapy and to explore the underlying mechanism. The finding indicated that PF and AF significantly increased the numbers of white blood cells (WBC) and reversed the atrophy of thymus. Furthermore, PF and AF increased the levels of granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-3 (IL-3) and reduced the levels of tumor necrosis factor-α (TNF-α) in serum and increased the level of colony-stimulating factor (G-CSF) in plasma. Lastly, PF and AF not only enhanced the mRNA levels of GM-CSF and G-CSF in the spleens, but also increased the protein levels of G-CSF and GM-CSF in bone marrow. Our results suggest that PF and AF may promote the recovery of bone marrow hemopoietic function in a myelosuppressed mouse model.

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Figures

Figure 1
Figure 1
Chemical structures and HPLC chromatograms of paeoniflorin (PF) (a) and albiflorin (AF) (b).
Figure 2
Figure 2
Effects of PF and AF on body weight, thymus index, and spleen index. Control = negative control (with same volume of physiologic saline). Data are expressed as means ± SD (n = 10). Compared with the control group: ## P < 0.01 and ### P < 0.001; compared with the model group: ∗ P < 0.05 and ∗∗ P < 0.01.
Figure 3
Figure 3
Effects of PF and AF on the bone marrow tissue histomorphology of femoral bone (H&E staining. (a): 1 × 200, (b): 1 × 400).
Figure 4
Figure 4
Effects of AF on GM-CSF, G-CSF, IL-3, IL-6, and TNF-α mRNA levels. Control = negative control (with same volume of physiologic saline). Data are expressed as means ± SD (n = 3). Compared with the control group: ## P < 0.01; compared with the model group: ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001.
Figure 5
Figure 5
Effects of PF and AF on GM-CSF, G-CSF, IL-3, IL-6, and TNF-α protein levels. Control = negative control (with same volume of physiologic saline). Data are expressed as means ± SD (n = 3). Compared with the control group: # P < 0.05 and ## P < 0.01; compared with the model group: ∗∗ P < 0.01 and ∗∗∗ P < 0.001.
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References

    1. Yeh L. L. L., Liu J.-Y., Liu Y.-S., Lin K.-S., Tsai T.-F., Wang L.-H. Anemia-related hemogram, uterine artery pulsatility index, and blood pressure for the effects of four-agents-decoction (Si Wu Tang) in the treatment of primary dysmenorrhea. Journal of Alternative and Complementary Medicine. 2009;15(5):531–538. doi: 10.1089/acm.2008.0208. - DOI - PubMed
    1. National Committee of Pharmacopoeia. Pharmacopoeia of the People's Republic of China (Part one) Beijing, China: Chemical Industry Press; 2015.
    1. Yu H.-Y., Mu D.-G., Chen J., Yin W. Suppressive effects of intrathecal paeoniflorin on bee venom-induced pain-related behaviors and spinal neuronal activation. Pharmacology. 2011;88(3-4):159–166. doi: 10.1159/000330456. - DOI - PubMed
    1. Jiang C., Xu L., Chen L., et al. Selective suppression of microglial activation by paeoniflorin attenuates morphine tolerance. European Journal of Pain. 2015;19(7):908–919. doi: 10.1002/ejp.617. - DOI - PubMed
    1. Nam K.-N., Yae C. G., Hong J.-W., Cho D.-H., Lee J. H., Lee E. H. Paeoniflorin, a monoterpene glycoside, attenuates lipopolysaccharide-induced neuronal injury and brain microglial inflammatory response. Biotechnology Letters. 2013;35(8):1183–1189. doi: 10.1007/s10529-013-1192-8. - DOI - PubMed

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