Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2016 Aug:127:160-163.
doi: 10.1016/j.mimet.2016.06.015. Epub 2016 Jun 14.

Variation in copy number of the 28S rDNA of Aspergillus fumigatus measured by droplet digital PCR and analog quantitative real-time PCR

Alexandre Alanio  1 , Aude Sturny-Leclère  2 , Marion Benabou  3 , Nicolas Guigue  4 , Stéphane Bretagne  5
Affiliations

Variation in copy number of the 28S rDNA of Aspergillus fumigatus measured by droplet digital PCR and analog quantitative real-time PCR

Alexandre Alanio et al. J Microbiol Methods. 2016 Aug.

Abstract

Droplet digital PCR (ddPCR) after DNA digestion yielded a 28S rDNA copy number of 61 to 86 copies/genome when testing 10 unrelated Aspergillus fumigatus isolates, higher than with quantitative PCR. Unfortunately, ddPCR after DNA digestion did not improve the sensitivity of our PCR assay when testing serum patients with invasive aspergillosis.

Keywords: Aspergillus fumigatus; Copy number variation; Droplet digital PCR; Real-time quantitative PCR; rDNA.

PubMed Disclaimer

MeSH terms

LinkOut - more resources