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Comparative Study
. 2017 May;22(3):119-126.
doi: 10.1080/13510002.2016.1198104. Epub 2016 Jun 20.

Parameters of oxidative stress in saliva from patients with aggressive and chronic periodontitis

Andrea B Acquier  1   2 , Alejandra K De Couto Pita  1 , Lucila Busch  1 , Gabriel A Sánchez  3
Affiliations
Comparative Study

Parameters of oxidative stress in saliva from patients with aggressive and chronic periodontitis

Andrea B Acquier et al. Redox Rep. 2017 May.

Abstract

Objectives: Free radicals play an important role in the onset and progression of many diseases. The aim of this study was to investigate the contribution of oxidative stress in the pathology of aggressive (AgP) and chronic (CP) periodontitis and its relation with the clinical periodontal status.

Methods: Eighty subjects were divided into two groups: 20 patients with AgP and 20 patients with CP with their 20 corresponding matched controls, based on clinical attachment loss (CAL), probing pocket depth (PPD), and bleeding on probing (BOP). Saliva reactive oxygen species (ROS), lipid peroxidation, and non-enzymatic antioxidant defences were measured by luminol-dependent chemiluminescence assay, as thiobarbituric acid-reactive substances (TBARs) and total radical-trapping antioxidant potential (TRAP), respectively. Pearson's correlation and multivariate analysis were used to determine the relationship between ROS and TBARs and the clinical parameters.

Results: ROS and TBARs were increased in AgP while TRAP was decreased, comparing with CP. In AgP, a strong and positive correlation was observed between ROS and TBARs and they were closely associated with CAL and PPD.

Discussion: In AgP, but not in CP, oxidative stress is a high contributor to periodontal pathology and it is closely associated with the clinical periodontal status.

Keywords: Aggressive periodontitis; Chronic periodontitis; Oxidative stress; ROS; TBARs; TRAP.

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Conflict of interest statement

Conflict of interest The authors have no conflict of interest to disclose.

Figures

Figure 1
Figure 1
(A) Generation of ROS expressed as RLU, in saliva from patients with aggressive (AgP) and chronic periodontal (CP) disease and their matched controls. (B) TRAP expressed as µM Trolox in saliva from patients with aggressive (AgP) and chronic (CP) periodontal disease and their matched controls. ***Significantly different from controls (P < 0.001); ###significantly different from AgP (P < 0.001). (C) Lipid peroxidation measured as TBARs (µM) in saliva from patients with aggressive (AgP) and chronic (CP) periodontal disease and their matched controls. ***Significantly different from controls (P < 0.001); **significantly different from controls (P < 0.01); ###significantly different from CP (P < 0.001).
Figure 2
Figure 2
Pearson correlation analysis between salivary levels of ROS and lipid peroxidation measured as salivary TBARs in AgP (A) and CP (B) groups.
Figure 3
Figure 3
Pearson correlation analysis between salivary levels of ROS and salivary protein concentration in AgP (A) and CP (B) groups.
Figure 4
Figure 4
Pearson correlation analysis between ROS and the clinical parameters PPD and the CAL in AgP (A) and CP (B) groups. ****Significant correlation (P < 0.0001). **Significant correlation (P < 0.01).
Figure 5
Figure 5
Pearson correlation analysis between lipid peroxidation measured as TBARs and the clinical parameters PPD and the CAL in AgP (A) and CP (B) groups. ****Significant correlation (P < 0.0001). *Significant correlation (P < 0.05).
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