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. 1989;91(4):315-9.
doi: 10.1007/BF00493007.

Cellular localization of induced human interferon-beta mRNA by non-radioactive in situ hybridization

H Multhaupt  1 , G Gross, P Fritz, K Köhler
Affiliations

Cellular localization of induced human interferon-beta mRNA by non-radioactive in situ hybridization

H Multhaupt et al. Histochemistry. 1989.

Abstract

Induced interferon-beta (IFN-beta) mRNA was localized in human FS-4 fibroblasts by in situ hybridization using biotinylated probes. The hybridization sites were detected by incubation with a nick-translated genomic DNA probe (1.8 kb) via streptavidin-colloidal gold followed by silver contrast enhancement. The positive signals were observed by reflection-contrast light microscopy. IFN-beta mRNA was transiently induced by poly r(I): r(C) in fibroblasts 2-4 h after induction. Induction in the presence of cycloheximide and actinomycin D (superinduction conditions) exhibited an enhanced level of IFN-beta mRNA with a maximum at 4-8 h. The kinetics of the IFN-beta mRNA expression in the cytoplasm as revealed by in situ hybridization proved to be compatible with the results of Northern blotting experiments of total cellular RNA.

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