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Comparative Study
. 1989 May;25(5):477-80.
doi: 10.1007/BF02624636.

An alternative method for the isolation of NS-1 hybridomas using cholesterol auxotrophy of NS-1 mouse myeloma cells

Y Myoken  1 , T Okamoto, T Osaki, M Yabumoto, G H Sato, K Takada, J D Sato
Affiliations
Comparative Study

An alternative method for the isolation of NS-1 hybridomas using cholesterol auxotrophy of NS-1 mouse myeloma cells

Y Myoken et al. In Vitro Cell Dev Biol. 1989 May.

Abstract

We have used the cholesterol auxotrophy of NS-1 mouse myeloma cells as the basis for selecting NS-1 hybridomas. The outgrowth of nascent NS-1 hybridomas in cholesterol-free serum-free medium was 3- to 9-fold more efficient than that in HAT medium and resulted in 3- to 13-times as many antigen-reactive hybridoma wells. This method of hybridoma selection can be applied with any sterol-dependent parent cell line. Hybridomas established under serum-free culture conditions were growth inhibited by fetal calf serum.

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