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. 2016 Jun 16;21(6):784.
doi: 10.3390/molecules21060784.

Ultrasound-Assisted Extraction of Stilbenes from Grape Canes

Zulema Piñeiro  1 , Almudena Marrufo-Curtido  2 , Maria Jose Serrano  3 , Miguel Palma  4
Affiliations

Ultrasound-Assisted Extraction of Stilbenes from Grape Canes

Zulema Piñeiro et al. Molecules. .

Abstract

An analytical ultrasound-assisted extraction (UAE) method has been optimized and validated for the rapid extraction of stilbenes from grape canes. The influence of sample pre-treatment (oven or freeze-drying) and several extraction variables (solvent, sample-solvent ratio and extraction time between others) on the extraction process were analyzed. The new method allowed the main stilbenes in grape canes to be extracted in just 10 min, with an extraction temperature of 75 °C and 60% ethanol in water as the extraction solvent. Validation of the extraction method was based on analytical properties. The resulting RSDs (n = 5) for interday/intraday precision were less than 10%. Furthermore, the method was successfully applied in the analysis of 20 different grape cane samples. The result showed that grape cane byproducts are potentially sources of bioactive compounds of interest for pharmaceutical and food industries.

Keywords: HPLC; extraction; grape canes; stilbenes; trans-resveratrol; ultrasound.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
HPLC-DAD chromatogram corresponding to Malbec cultivar grape cane sample and structure of the studied compounds.
Figure 2
Figure 2
Total stilbenes in different assayed drying processes of grape canes. Bars with * symbol are significantly different at significance levels. * p < 0.05, ** p < 0.01 and *** p < 0.001.
See this image and copyright information in PMC

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