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Case Reports
. 2016 Sep;22(9):1647-9.
doi: 10.3201/eid2209.160132. Epub 2016 Sep 15.

Mutation in West Nile Virus Structural Protein prM during Human Infection

Yaniv Lustig, Robert S Lanciotti, Musa Hindiyeh, Nathan Keller, Ron Milo, Shlomo Mayan, Ella Mendelson
Case Reports

Mutation in West Nile Virus Structural Protein prM during Human Infection

Yaniv Lustig et al. Emerg Infect Dis. 2016 Sep.

Abstract

A mutation leading to substitution of a key amino acid in the prM protein of West Nile virus (WNV) occurred during persistent infection of an immunocompetent patient. WNV RNA persisted in the patient's urine and serum in the presence of low-level neutralizing antibodies. This case demonstrates active replication of WNV during persistent infection.

Keywords: WNV; WNV mutation; West Nile virus; human infection; persistent infection; prM protein; viruses.

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Figures

Figure
Figure
Phylogenetic analysis of West Nile virus (WNV) lineage 1 isolate from a patient with persistent WNV infection, Israel, 2014, compared with reference strains. The analysis was conducted on 96% of the WNV nucleotide sequence using the neighbor-joining method implemented in MEGA 6.0 software (http://www.megasoftware.net). The robustness of branching pattern was tested by 1,000 bootstrap replications. The percentage of successful bootstrap replicates is indicated at nodes, showing only values of >70%. A WNV lineage 2 sequence obtained from strain ITA 13 (GenBank no. KF647252) was used as an outgroup. Bold indicates the WNV lineage 1 strain sequenced in this study Scale bar indicates nucleotide substitutions per site.
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