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. 2016 Aug 22;60(9):5589-91.
doi: 10.1128/AAC.00935-16. Print 2016 Sep.

Full-Length Nucleotide Sequences of mcr-1-Harboring Plasmids Isolated from Extended-Spectrum-β-Lactamase-Producing Escherichia coli Isolates of Different Origins

Katrin Zurfluh  1 , Jochen Klumpp  2 , Magdalena Nüesch-Inderbinen  3 , Roger Stephan  3
Affiliations

Full-Length Nucleotide Sequences of mcr-1-Harboring Plasmids Isolated from Extended-Spectrum-β-Lactamase-Producing Escherichia coli Isolates of Different Origins

Katrin Zurfluh et al. Antimicrob Agents Chemother. .

Abstract

Here, we present the full sequences of three mcr-1-carrying plasmids isolated from extended-spectrum-β-lactamase (ESBL)-producing Escherichia coli The plasmids belong to three different replicon types and are 34,640 bp, 209,401 bp, and 247,885 bp in size. We describe for the first time a composite transposon containing mcr-1 localized on a multidrug-resistant (MDR) IncHI2 plasmid harboring additional determinants of resistance to six different classes of antibiotics, including the ESBL gene blaCTX-M-1, and heavy metal resistance.

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Figures

FIG 1
FIG 1
Genetic structures surrounding the mcr-1 gene in plasmids of this study compared to those in plasmid pHNSHP45. The arrows indicate open reading frames (orf), with black, stippled, gray, and white arrows representing mcr-1, insertion (IS) elements, ORFs with known function, and ORFs with unknown function, respectively.
See this image and copyright information in PMC

References

    1. Liu YY, Wang Y, Walsh TR, Yi LX, Zhang R, Spencer J, Doi Y, Tian G, Dong B, Huang X, Yu LF, Gu D, Ren H, Chen X, Lv L, He D, Zhou H, Liang Z, Liu JH, Shen J. 2016. Emergence of plasmid-mediated colistin resistance mechanism MCR-1 in animals and human beings in China: a microbiological and molecular biological study. Lancet Infect Dis 16:161–168. doi:10.1016/S1473-3099(15)00424-7. - DOI - PubMed
    1. Shen Z, Wang Y, Shen Y, Shen J, Wu C. 2016. Early emergence of mcr-1 in Escherichia coli from food-producing animals. Lancet Infect Dis 16:293. doi:10.1016/S1473-3099(16)00061-X. - DOI - PubMed
    1. Falgenhauer L, Waezsada SE, Yao Y, Imirzalioglu C, Käsbohrer A, Roesler U, Michael GB, Schwarz S, Werner G, Kreienbock L, Chakraborty T, for the RESET consortium. 2016. Colistin resistance gene mcr-1 in extended-spectrum beta-lactamase-producing and carbapenemase-producing Gram-negative bacteria in Germany. Lancet Infect Dis 16:282–283. doi:10.1016/S1473-3099(16)00009-8. - DOI - PubMed
    1. Zhi C, Lv L, Yu LF, Doi Y, Liu JH. 2016. Dissemination of the mcr-1 colistin resistance gene. Lancet Infect Dis 16:292–293. doi:10.1016/S1473-3099(16)00063-3. - DOI - PubMed
    1. Nordmann P, Lienhard R, Kieffer N, Clerc O, Poirel L. 2016. Plasmid-mediated colistin-resistant Escherichia coli in bacteremia in Switzerland. Clin Infect Dis 15:1322–1323. doi:10.1093/cid/ciw124. - DOI - PubMed

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