Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1978 Feb;75(2):901-4.
doi: 10.1073/pnas.75.2.901.

Growth and maintenance of HeLa cells in serum-free medium supplemented with hormones

S E Hutchings, G H Sato

Growth and maintenance of HeLa cells in serum-free medium supplemented with hormones

S E Hutchings et al. Proc Natl Acad Sci U S A. 1978 Feb.

Abstract

HeLa cells grow in a nutritionally complete synthetic medium (Ham's F12) supplemented with insulin, transferrin, hydrocortisone (aldosterone), fibroblast growth factor, and epidermal growth factor. This hormone-supplemented medium supports clonal growth, long-term cultivation, and a growth rate equal to that of serum-supplemented medium. The omission of any one of the five components results in less than maximal cell growth.

PubMed Disclaimer

References

    1. Proc Natl Acad Sci U S A. 1955 Jul 15;41(7):432-7 - PubMed
    1. J Biol Chem. 1959 Oct;234:2754-8 - PubMed
    1. Science. 1955 Sep 16;122(3168):501-14 - PubMed
    1. Proc Natl Acad Sci U S A. 1965 Feb;53:288-93 - PubMed
    1. Exp Cell Res. 1964 Dec;36:439-74 - PubMed

Publication types

LinkOut - more resources