. 2016 Sep;54(9):2380-3.

doi: 10.1128/JCM.00982-16. Epub 2016 Jun 22.

Duplex Quantitative PCR Assay for Detection of Haemophilus influenzae That Distinguishes Fucose- and Protein D-Negative Strains

Camilla de Gier¹, Janessa L Pickering², Peter C Richmond³, Ruth B Thornton², Lea-Ann S Kirkham⁴

Affiliations

¹ School of Paediatrics and Child Health, University of Western Australia, Perth, Australia.
² School of Paediatrics and Child Health, University of Western Australia, Perth, Australia Wesfarmers Centre for Vaccines and Infectious Diseases, Telethon Kids Institute, Perth, Australia.
³ School of Paediatrics and Child Health, University of Western Australia, Perth, Australia Wesfarmers Centre for Vaccines and Infectious Diseases, Telethon Kids Institute, Perth, Australia Department of Paediatrics, Princess Margaret Hospital for Children, Subiaco, Australia.
⁴ School of Paediatrics and Child Health, University of Western Australia, Perth, Australia Wesfarmers Centre for Vaccines and Infectious Diseases, Telethon Kids Institute, Perth, Australia lea-ann.kirkham@uwa.edu.au.

PMID: 27335148
PMCID: PMC5005495
DOI: 10.1128/JCM.00982-16

Duplex Quantitative PCR Assay for Detection of Haemophilus influenzae That Distinguishes Fucose- and Protein D-Negative Strains

Camilla de Gier et al. J Clin Microbiol. 2016 Sep.

. 2016 Sep;54(9):2380-3.

doi: 10.1128/JCM.00982-16. Epub 2016 Jun 22.

Authors

Camilla de Gier¹, Janessa L Pickering², Peter C Richmond³, Ruth B Thornton², Lea-Ann S Kirkham⁴

Affiliations

¹ School of Paediatrics and Child Health, University of Western Australia, Perth, Australia.
² School of Paediatrics and Child Health, University of Western Australia, Perth, Australia Wesfarmers Centre for Vaccines and Infectious Diseases, Telethon Kids Institute, Perth, Australia.
³ School of Paediatrics and Child Health, University of Western Australia, Perth, Australia Wesfarmers Centre for Vaccines and Infectious Diseases, Telethon Kids Institute, Perth, Australia Department of Paediatrics, Princess Margaret Hospital for Children, Subiaco, Australia.
⁴ School of Paediatrics and Child Health, University of Western Australia, Perth, Australia Wesfarmers Centre for Vaccines and Infectious Diseases, Telethon Kids Institute, Perth, Australia lea-ann.kirkham@uwa.edu.au.

PMID: 27335148
PMCID: PMC5005495
DOI: 10.1128/JCM.00982-16

Abstract

We have developed a specific Haemophilus influenzae quantitative PCR (qPCR) that also identifies fucose-negative and protein D-negative strains. Analysis of 100 H. influenzae isolates, 28 Haemophilus haemolyticus isolates, and 14 other bacterial species revealed 100% sensitivity (95% confidence interval [CI], 96% to 100%) and 100% specificity (95% CI, 92% to 100%) for this assay. The evaluation of 80 clinical specimens demonstrated a strong correlation between semiquantitative culture and the qPCR (P < 0.001).

PubMed Disclaimer

Cited by

Patients with Chronic Obstructive Pulmonary Disease harbour a variation of Haemophilus species.
Osman KL, Jefferies JMC, Woelk CH, Devos N, Pascal TG, Mortier MC, Devaster JM, Wilkinson TMA, Cleary DW, Clarke SC; AERIS Study Group. Osman KL, et al. Sci Rep. 2018 Oct 3;8(1):14734. doi: 10.1038/s41598-018-32973-3. Sci Rep. 2018. PMID: 30282975 Free PMC article. Clinical Trial.
Haemophilus influenzae persists in biofilm communities in a smoke-exposed ferret model of COPD.
Hunt BC, Stanford D, Xu X, Li J, Gaggar A, Rowe SM, Raju SV, Swords WE. Hunt BC, et al. ERJ Open Res. 2020 Aug 11;6(3):00200-2020. doi: 10.1183/23120541.00200-2020. eCollection 2020 Jul. ERJ Open Res. 2020. PMID: 32802827 Free PMC article.
Sleep Disordered Breathing and Recurrent Tonsillitis Are Associated With Polymicrobial Bacterial Biofilm Infections Suggesting a Role for Anti-Biofilm Therapies.
Mateus T, Seppanen EJ, de Gier C, Clark S, Coates H, Vijayasekaran S, Prosser K, Wiertsema SP, Fuery A, Kirkham LS, Richmond PC, Thornton RB. Mateus T, et al. Front Cell Infect Microbiol. 2022 Feb 28;12:831887. doi: 10.3389/fcimb.2022.831887. eCollection 2022. Front Cell Infect Microbiol. 2022. PMID: 35295756 Free PMC article.
Australian Aboriginal Children with Otitis Media Have Reduced Antibody Titers to Specific Nontypeable Haemophilus influenzae Vaccine Antigens.
Thornton RB, Kirkham LS, Corscadden KJ, Wiertsema SP, Fuery A, Jones BJ, Coates HL, Vijayasekaran S, Zhang G, Keil A, Richmond PC. Thornton RB, et al. Clin Vaccine Immunol. 2017 Apr 5;24(4):e00556-16. doi: 10.1128/CVI.00556-16. Print 2017 Apr. Clin Vaccine Immunol. 2017. PMID: 28151410 Free PMC article.
Direct Real-Time PCR for the Detection and Serotyping of Haemophilus influenzae without DNA Extraction.
Marasini D, Whaley MJ, Jenkins LT, Hu F, Jiang W, Topaz N, Chen A, Schmink S, Dolan Thomas J, Harcourt BH, Marjuki H, Wang X. Marasini D, et al. J Clin Microbiol. 2022 Apr 20;60(4):e0211121. doi: 10.1128/jcm.02111-21. Epub 2022 Mar 21. J Clin Microbiol. 2022. PMID: 35306833 Free PMC article.

See all "Cited by" articles

References

1. Agrawal A, Murphy TF. 2011. Haemophilus influenzae infections in the H. influenzae type b conjugate vaccine era. J Clin Microbiol 49:3728–3732. doi:10.1128/JCM.05476-11. - DOI - PMC - PubMed
1. Brook I, Foote PA, Hausfeld JN. 2006. Frequency of recovery of pathogens causing acute maxillary sinusitis in adults before and after introduction of vaccination of children with the 7-valent pneumococcal vaccine. J Med Microbiol 55:943–946. doi:10.1099/jmm.0.46346-0. - DOI - PubMed
1. Coker TR, Chan LS, Newberry SJ, Limbos MA, Suttorp MJ, Shekelle PG, Takata GS. 2010. Diagnosis, microbial epidemiology, and antibiotic treatment of acute otitis media in children: a systematic review. JAMA 304:2161–2169. doi:10.1001/jama.2010.1651. - DOI - PubMed
1. Sethi S, Murphy TF. 2008. Infection in the pathogenesis and course of chronic obstructive pulmonary disease. N Engl J Med 359:2355–2365. doi:10.1056/NEJMra0800353. - DOI - PubMed
1. Leibovitz E, Jacobs MR, Dagan R. 2004. Haemophilus influenzae: a significant pathogen in acute otitis media. Pediatr Infect Dis J 23:1142–1152. - PubMed

MeSH terms

Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions

Substances

Actions
Actions
Actions
Actions
Actions
Actions

LinkOut - more resources

Full Text Sources
Other Literature Sources
- scite Smart Citations
Molecular Biology Databases
- BacDive

Save citation to file

Email citation

Add to Collections

Add to My Bibliography

Your saved search

Create a file for external citation management software

Your RSS Feed

Duplex Quantitative PCR Assay for Detection of Haemophilus influenzae That Distinguishes Fucose- and Protein D-Negative Strains

Affiliations

Duplex Quantitative PCR Assay for Detection of Haemophilus influenzae That Distinguishes Fucose- and Protein D-Negative Strains

Authors

Affiliations

Abstract

Similar articles

Cited by

References

MeSH terms

Substances

LinkOut - more resources

Full Text Sources

Other Literature Sources

Molecular Biology Databases

Abstract

Similar articles

Cited by

References

MeSH terms

Substances

Related information

LinkOut - more resources

Full Text Sources

Other Literature Sources

Molecular Biology Databases