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Comment
. 2016 Jun 27:5:e18203.
doi: 10.7554/eLife.18203.

Falling apart

Jonathan S Marvin  1 , Loren L Looger  1
Affiliations
Comment

Falling apart

Jonathan S Marvin et al. Elife. .

Abstract

Destabilized nanobodies can be used to deliver fluorescent proteins and enzymes to specific targets inside cells.

Keywords: GFP; HIV-1; antibody engineering; developmental biology; human; mouse; nanobodies; neuroscience; stem cells.

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Conflict of interest statement

The authors declare that no competing interests exist.

Figures

Figure 1.
Figure 1.. Using destabilized nanobodies for the targeted labeling or manipulation of proteins within live cells.
The ability of an antibody to target a specific molecule can be exploited in a range of cell biology experiments, but expressing antibodies in living cells (top) can be difficult. Using a destabilized nanobody (dNb; blue) can overcome some of these problems (Tang et al., 2016). Destabilized nanobodies can be used to deliver fluorescent labels or specific enzymes (green cylinders) to specific targets (red; right). If the target is already saturated by nanobody, the rest of the nanobody is degraded (left), thus preventing unwanted background fluorescence or enzyme activity.
See this image and copyright information in PMC

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