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. 2016 Mar;16(2):300-3.
doi: 10.1016/j.scr.2015.12.026. Epub 2016 Jan 4.

Generation of iPSC line iPSC-FH2.1 in hypoxic conditions from human foreskin fibroblasts

María Questa  1 , Leonardo Romorini  2 , Carolina Blüguermann  3 , Claudia María Solari  4 , Gabriel Neiman  3 , Carlos Luzzani  3 , María Élida Scassa  3 , Gustavo Emilio Sevlever  3 , Alejandra Sonia Guberman  5 , Santiago Gabriel Miriuka  2
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Free article

Generation of iPSC line iPSC-FH2.1 in hypoxic conditions from human foreskin fibroblasts

María Questa et al. Stem Cell Res. 2016 Mar.
Free article

Abstract

Human foreskin fibroblasts were used to generate the iPSC line iPSC-FH2.1 using the EF1a-hSTEMCCA-loxP vector expressing OCT4, SOX2, c-MYC and KLF4, in 5% O2 culture conditions. Stemness was confirmed, as was pluripotency both in vivo and in vitro, in normoxia and hypoxia. Human Embryonic Stem Cell (hESC) line WA-09 and reprogrammed fibroblast primary culture HFF-FM were used as controls.

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