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. 2016 May 15;8(5):2438-49.
eCollection 2016.

Bioinformatics investigation of therapeutic mechanisms of Xuesaitong capsule treating ischemic cerebrovascular rat model with comparative transcriptome analysis

Jiangquan Liao  1 , Benjun Wei  2 , Hengwen Chen  3 , Yongmei Liu  3 , Jie Wang  3
Affiliations

Bioinformatics investigation of therapeutic mechanisms of Xuesaitong capsule treating ischemic cerebrovascular rat model with comparative transcriptome analysis

Jiangquan Liao et al. Am J Transl Res. .

Abstract

Background: Xuesaitong soft capsule (XST) which consists of panax notoginseng saponin (PNS) has been used to treat ischemic cerebrovascular diseases in China. The therapeutic mechanism of XST has not been elucidated yet from prospective of genomics and bioinformatics.

Methods: A transcriptome analysis was performed to review series concerning middle cerebral artery occlusion (MCAO) rat model and XST intervention after MCAO from Gene Expression Omnibus (GEO) database. Differentially expressed genes (DEGs) were compared between blank group and model group, model group and XST group. Functional enrichment and pathway analysis were performed. Protein-Protein interaction network was constructed. The overlapping genes from two DEGs sets were screened out and profound analysis was performed.

Results: Two series including 22 samples were obtained. 870 DEGs were identified between blank group and model group, and 1189 DEGs were identified between model group and XST group. GO terms and KEGG pathways of MCAO and XST intervention were significantly enriched. PPI networks were constructed to demonstrate the gene-gene interactions. The overlapping genes from two DEGs sets were highlighted. ANTXR2, FHL3, PRCP, TYROBP, TAF9B, FGFR2, BCL11B, RB1CC1 and MBNL2 were the pivotal genes and possible action sites of XST therapeutic mechanisms.

Conclusion: MCAO is a pathological process with multiple.

Keywords: Middle cerebral artery occlusion; Traditional Chinese medicine; Xuesaitong capsule; bioinformatics; transcriptome analysis.

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Figures

Figure 1
Figure 1
PPI network of normal group vs model group. The upregulated DEGs are in red and those downregulated are in green. The more one node interacts with others, the lager it is.
Figure 2
Figure 2
PPI network of model group vs XST group. The upregulated DEGs are in red and those downregulated are in green. The more one node interacts with others, the lager it is.
See this image and copyright information in PMC

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