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. 2016 Jun 29:13:115.
doi: 10.1186/s12985-016-0571-x.

Isolation of an imported subgenotype B5 strain of human enterovirus A71 in Chongqing City, China, 2014

Qian Yang  1 , Yong Zhang  1 , Dongmei Yan  1 , Shuangli Zhu  1 , Dongyan Wang  1 , Tianjiao Ji  1 , Wei Huang  2 , Hongqiu An  1 , Wenbo Xu  3
Affiliations

Isolation of an imported subgenotype B5 strain of human enterovirus A71 in Chongqing City, China, 2014

Qian Yang et al. Virol J. .

Abstract

Background: Enterovirus A71 (EV-A71) is the main pathogen responsible for large outbreaks of hand, foot, and mouth disease (HFMD) in mainland China, and the dominant EV-A71 strains belong to subgenotype C4. To date, only one imported subgenotype B5 of EV-A71 has been reported in Xiamen City Fujian Province, 2009.

Results: Here, we report on another imported subgenotype B5 of EV-A71 isolated from a HFMD patient in Chongqing City in 2014 (strain CQ2014-86/CQ/CHN/2014, hereafter refer as CQ2014-86). The VP1 coding sequence and the whole genome sequence revealed that strain CQ2014-86 shares the high nucleotide identity with Vietnamese strains isolated in 2011-2013, suggesting that strain CQ2014-86 may have been imported from Vietnam. In the 5'UTR, P2 and P3 regions, recombination events were found between strain CQ2014-86 and other EV-A, such as coxsackievirus A4 (CV-A4), CV-A5, CV-A14 and CV-A16.

Conclusions: This is the second report on importation of subgenotype B5 of EV-A71 in China, implying that we need to pay more attention to the importation of different subgenotypes of EV-A71.

Keywords: Human enterovirus A71; Importation; Subgenotype B5.

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Figures

Fig. 1
Fig. 1
a Phylogenetic tree based on the entire VP1 coding region (891 bp) of subgenotype B5 of EV-A71. Black triangle indicated Chongqing strain KU647000-CQ2014-86/CQ/CHN/2014. Black round indicated Xiamen strain JN964686-Xiamen/FJ/CHN/2009. Strain CQ2014-86 and reference strains from GenBank database of subgenotype B5 of EV-A71 were included. Neighbor-joining algorithm was used for phylogenetic analysis with Mega software (version5.03). b Phylogenetic tree based on the complete genome of subgenotype B5 of EV-A71. Black triangle indicated Chongqing strain KU647000-CQ2014-86/CQ/CHN/2014. Black round indicated Xiamen strain JN964686-Xiamen/FJ/CHN/2009. The prototype of EV-A71(BrCr), CV-A16(G-10) and the oldest sequences of different subgenotypes of EV-A71 (B0-5, C1-6 subgenotypes) were included as well
Fig. 2
Fig. 2
Similarity plot and bootscanning analyses of the whole genome of the EV-A strains. a Similarity plot and b bootscanning analysis. A sliding window of 200 nucleotides was used, moving in 20 nucleotide steps. The Chongqing strain CQ2014-86/CQ/CHN/2014 was used as the query sequence. c Similarity plot and d bootscanning analysis. A sliding window of 200 nucleotides was used, moving in 20 nucleotide steps. The Chongqing strain JN964686-Xiamen/FJ/CHN/2009 was used as the query sequence
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References

    1. Tan X, Huang X, Zhu S, Chen H, Yu Q, Wang H, Huo X, Zhou J, Wu Y, Yan D, et al. The persistent circulation of enterovirus 71 in People’s Republic of China: causing emerging nationwide epidemics since 2008. PLoS One. 2011;6:e25662. doi: 10.1371/journal.pone.0025662. - DOI - PMC - PubMed
    1. Zhang Y, Tan X, Wang H, Yan D, Zhu S, Wang D, Ji F, Wang X, Gao Y, Chen L, et al. An outbreak of hand, foot, and mouth disease associated with subgenotype C4 of human enterovirus 71 in Shandong. China J Clin Virol. 2009;44:262–7. doi: 10.1016/j.jcv.2009.02.002. - DOI - PubMed
    1. Zhang Y, Zhu Z, Yang W, Ren J, Tan X, Wang Y, Mao N, Xu S, Zhu S, Cui A, et al. An emerging recombinant human enterovirus 71 responsible for the 2008 outbreak of hand foot and mouth disease in Fuyang city of China. Virol J. 2010;7:94. doi: 10.1186/1743-422X-7-94. - DOI - PMC - PubMed
    1. Cui A, Xu C, Tan X, Zhang Y, Zhu Z, Mao N, Lu Y, Xu W. The development and application of the two real-time RT-PCR assays to detect the pathogen of HFMD. PLoS One. 2013;8:e61451. doi: 10.1371/journal.pone.0061451. - DOI - PMC - PubMed
    1. Zhang Y, Tan X, Cui A, Mao N, Xu S, Zhu Z, Zhou J, Shi J, Zhao Y, Wang X, et al. Complete genome analysis of the C4 subgenotype strains of enterovirus 71: predominant recombination C4 viruses persistently circulating in China for 14 years. PLoS One. 2013;8:e56341. doi: 10.1371/journal.pone.0056341. - DOI - PMC - PubMed

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