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. 2016 Jul;41(4):334-9.

miR-4284 and miR-4484 as Putative Biomarkers for Diffuse Large B-Cell Lymphoma

Gholamhossein Tamaddon  1 , Bita Geramizadeh  2 , Mohammad Hossein Karimi  3 , Seyed Javad Mowla  4 , Said Abroun  1
Affiliations

miR-4284 and miR-4484 as Putative Biomarkers for Diffuse Large B-Cell Lymphoma

Gholamhossein Tamaddon et al. Iran J Med Sci. 2016 Jul.

Abstract

Diffuse large B-cell lymphoma is the most common type of non-Hodgkin lymphoma. MicroRNAs (miRNAs) are endogenous small RNA, which can regulate gene expression at the post-transcriptional level. MiRNA profiling has shown a great potential as novel diagnostic and prognostic biomarkers. The present study was performed at the Nemazee Teaching Hospital (Shiraz, Iran) from 2011 to 2013. The aim of this study was to assess the deregulation of miRNAs profiles in DLBL against hyperplasic reactive lymph node as a normal. This could serve as a biomarker for DLBL. The miRCURY LNA™ microarray was used on the total RNA, which was extracted from formalin-fixed paraffin-embedded tissue of 24 de novo diffuse large B-cell lymphoma patients and 14 normal lymph nodes. The greatest changes were detected in miR-4284 and miR-4484 level in patient's lymphoma samples. These miRNAs can act as a diagnostic biomarker for DLBL.

Keywords: Diffuse; Large B-Cell; Lymphoma; MicroRNAs; Microarray Analysis.

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Figures

Figure 1
Figure 1
M/A plot show normalization by the use of spike-ins and LOWESS. Green: Hy3 controls; Red: Spike-ins; Black: All other probes.
Figure 2
Figure 2
The principal component analysis performed on all samples and on the top 50 microRNAs of DLBL and normal.
Figure 3
Figure 3
Heat map and unsupervised hierarchical clustering show miRNAs micro array of DLBL against normal samples. Red color display an expression below the reference channel and green color display the expression higher than the reference channel.
Figure 4
Figure 4
The most alteration shows microRNAs expressions of DLBL patients against normal.
See this image and copyright information in PMC

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