Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2001 Jul;57(3):188-90.
doi: 10.1016/S0377-1237(01)80039-3. Epub 2011 Jul 21.

IMMUNOCHROMATOGRAPHIC TEST: A NEW DIMENSION IN DIAGNOSIS OF PLASMODIUM FALCIPARUM MALARIA

M K Gupta  1 , R N Misra  2 , N Chawla  2 , H Mani  2 , C N Chowdhry  3 , S P Singh  4 , S Gupta  4
Affiliations

IMMUNOCHROMATOGRAPHIC TEST: A NEW DIMENSION IN DIAGNOSIS OF PLASMODIUM FALCIPARUM MALARIA

M K Gupta et al. Med J Armed Forces India. 2001 Jul.

Abstract

75 patients with clinical features suggestive of malaria were studied to evaluate the efficacy of immunochromatographic test (ICT), which detects histidine rich protein-2 antigen secreted by Plasmodium falciparum (Pfhrp-2), as against direct microscopy. There were 40 cases of P falciparum malaria, 14 cases of P vivax malaria and 21 cases of non-malarial fevers. Direct microscopy could detect 27(67.5%) P falciparum cases but failed to detect 13 cases (32.5%) whereas ICT could detect 35(87.5%) P falciparum cases out of 40 but failed to detect 5(12.5%) cases. All the P vivax cases and non-malarial fever cases were negative for ICT. The sensitivity and specificity of ICT is 87.5% and 100% respectively where as the positive predictive value and the negative predictive value of the test is 100% and 87.5% respectively. It is concluded that ICT test is a good adjunct to blood smear studies in fever cases with neurological and multiorgan dysfunction and in antenatal ladies.

Keywords: ICT; P falciparum.

PubMed Disclaimer

References

    1. Leke RF, Djokm RR, Leke RJ. Detection of Plasmodium falciparum antigen histidine rich protein-2 in the blood of pregnant women: implication diagnosis in placental malaria. J Clin Microbiol. 1999;37(9):2292–2296. - PMC - PubMed
    1. Neva FA. Observation on induced malaria: looking back for a view of the future. Am J Trop Med Hyg. 1997;26(S):211–216. - PubMed
    1. Howard RJ, Uni S, Aikawa M, Aley SB. Excretion of a malarial histidine rich protein-II from Plasmodium falciparum infected erythrocytes. J Cell Biol. 1986;103:1269–1277. - PMC - PubMed
    1. Wellems TE, Howard RJ. Homologous genes encode the distinct histidine rich protein in a cloned isolate of Plasmodium falciparum. Proc Natl Acad: Sci USA. 1983:6065–6069. - PMC - PubMed
    1. Mishra B, Samantaray JC, Nirdha BR. Evaluation of rapid antigen capture assay for the diagnosis of falciparum malaria. Ind J Med Res. 1999;109:16–19. - PubMed

Uncited Reference

    1. Kar I, Eapen A, Adak T, Sharma VP. Trial with ParaSight-F in the detection of Plasmodium falciparum infections in Chennai. Indian J Malariol. 1998;35(3):160–162. - PubMed

LinkOut - more resources