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. 2016 Feb 22;9(1):e3919.
doi: 10.17795/ijcp-3919. eCollection 2016 Feb.

Autophagy Gene Activity May Act As a Key Factor for Sensitivity of Tumor Cells to Oncolytic Vesicular Stomatitis Virus

Arezoo Nabizadeh  1 , Taravat Bamdad  1 , Ehsan Arefian  2 , Seyed Hadi Razavi Nikoo  3
Affiliations

Autophagy Gene Activity May Act As a Key Factor for Sensitivity of Tumor Cells to Oncolytic Vesicular Stomatitis Virus

Arezoo Nabizadeh et al. Iran J Cancer Prev. .

Abstract

Background: Beclin1 is an important, primary molecule for autophagy.

Objectives: It is suggested that the control of the autophagy path increases the sensitivity of tumor cells to VSV.

Materials and methods: In this study, the degree of Beclin1 gene expression in two cell lines, HeLa and A549, has been examined and the percentage of living cells subsequent infection with virus has been evaluated by MTT assay method.

Results: The results showed that the degree of Beclin1 gene expression in HeLa cells in comparison with A549 cells has reduced, and the sensitivity of these cells to vesicular stomatits virus (VSV) oncolysis is more than A549.

Conclusions: It seems that by using some methods for reducing autophagy, it is possible to make tumor cells more sensitive to virotherapy and even other treatments.

Keywords: Autophagy; Beclin1; Vesicular Stomatitis Virus; Virotherapy.

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Conflict of interest statement

Funding/Support:None declared.

Figures

Figure 1.
Figure 1.. The Ratio of Beclin1 Gene Expression in HeLa and A549 Cell Lines After Infection with VSV in Two Different MOIs, 0.1 and 0.001, Compared to Uninfected Cells, 12 Hours After Infection
Figure 2.
Figure 2.. The Percentage of Vital Cells in Different MOIs, of 1, 0.1, 0.01 and 0.001 Compared to Uninfected Cells, 24 Hours After Infection
See this image and copyright information in PMC

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