Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2016 Jun 18;9(6):816-20.
doi: 10.18240/ijo.2016.06.04. eCollection 2016.

Histopathological changes in retinas and F-ERG features of streptozotocin-induced diabetic rats treated with ozone

Ting-Yu Xie  1 , Qin Li  1 , Xue-Yi Chen  1
Affiliations

Histopathological changes in retinas and F-ERG features of streptozotocin-induced diabetic rats treated with ozone

Ting-Yu Xie et al. Int J Ophthalmol. .

Abstract

Aim: To study the histopathological changes in the retina and flash electroretinogram (F-ERG) features of ozone-treated streptozotocin (STZ)-induced diabetic rats.

Methods: Seventy male Sprague Dawley rats were grouped as follows: blank group (GB, n=10), model control group (GM, n=18), ozone group (GO3, n=19), and oxygen group (GO2, n=18). The model was induced by single intraperitoneal injection of STZ. Ozone or oxygen enteroclysm was given twice per week for 4wk. F-ERG and histopathological examinations were performed one month after treatment.

Results: Under dark adaption, as compared to GB, the other groups each had differential decreases in the a-wave amplitudes (P<0.05); the latencies were delayed in GM, GO2, and GO3 rats (P<0.05). Similar results were observed under light adaption, with the exception that the a-wave of the amplitudes (F=0.28, P>0.05). There were significant differences in the apoptosis index among the groups (P<0.05). Under ozone treatment, apoptosis was decreased in GO3 as compared to GM and GO2.

Conclusion: Ozone administration alleviates nerve damage and reduces pathology and apoptosis in the retinas of diabetic rats.

Keywords: diabetic rat retina; flash electroretinogram features; histopathological changes; ozone treatment.

PubMed Disclaimer

Figures

Figure 1
Figure 1. Apoptosis of the retina cells (arrows) in each group by TUNEL
A: Infrequent occurrence of TUNEL positive cells in GB; B: TUNEL positive cells seen in GCL and INL in the GM group; C: In GO2, TUNEL positive cells was as the same as GM; D: In GO3 group, a few TUNEL positive cells were seen in GCL and INL, more than in GB but less than in GO2 and GM (×400).
See this image and copyright information in PMC

References

    1. Ferris FL, 3rd, Davis MD, Aiello LM. Treatment of diabetic retinopathy. N Engl J Med. 1999;341(9):667–678. - PubMed
    1. Zheng Y, He M, Congdon N. The worldwide epidemic of diabetic retinopathy. Indian J Ophthalmol. 2012;60(5):428–431. - PMC - PubMed
    1. Bocci V. Oxygen-ozone therapy. A critical evaluation. London: akaluwer ED; 2002.
    1. Paoloni M, Di Sante L, Cacchio A, Apuzzo D, Marotta S, Razzano M, Franzini M, Santilli V. Intramuscular oxygen-ozone therapy in the treatment of acute back pain with lumbar disc herniation: a multicenter, randomized, double-blind, clinical trial of active and simulated lumbar paravertebral injection. Spine (Phila Pa 1976) 2009;34(13):1337–1344. - PubMed
    1. A SR, Reddy N, Dinapadu S, Reddy M, Pasari S. Role of ozone therapy in minimal intervention dentistry and endodontics-a review. J Int Oral Health. 2013;5(3):102–108. - PMC - PubMed

LinkOut - more resources