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. 2016:2016:2912418.
doi: 10.1155/2016/2912418. Epub 2016 Jun 6.

Effect of High Dietary Tryptophan on Intestinal Morphology and Tight Junction Protein of Weaned Pig

Myrlene Carine B Tossou  1 , Hongnan Liu  2 , Miaomiao Bai  2 , Shuai Chen  2 , Yinghua Cai  3 , Veeramuthu Duraipandiyan  4 , Hongbin Liu  3 , Tolulope O Adebowale  5 , Naif Abdullah Al-Dhabi  4 , Lina Long  2 , Hussain Tarique  1 , Abimbola O Oso  6 , Gang Liu  2 , Yulong Yin  1
Affiliations

Effect of High Dietary Tryptophan on Intestinal Morphology and Tight Junction Protein of Weaned Pig

Myrlene Carine B Tossou et al. Biomed Res Int. 2016.

Erratum in

Abstract

Tryptophan (Trp) plays an essential role in pig behavior and growth performances. However, little is known about Trp's effects on tight junction barrier and intestinal health in weaned pigs. In the present study, twenty-four (24) weaned pigs were randomly assigned to one of the three treatments with 8 piglets/treatments. The piglets were fed different amounts of L-tryptophan (L-Trp) as follows: 0.0%, 0.15, and 0.75%, respectively, named zero Trp (ZTS), low Trp (LTS), and high Trp (HTS), respectively. No significant differences were observed in average daily gain (ADG), average daily feed intake (ADFI), and gain: feed (G/F) ratio between the groups. After 21 days of the feeding trial, results showed that dietary Trp significantly increased (P < 0.05) crypt depth and significantly decreased (P < 0.05) villus height to crypt depth ratio (VH/CD) in the jejunum of pig fed HTS. In addition, pig fed HTS had higher (P < 0.05) serum diamine oxidase (DAO) and D-lactate. Furthermore, pig fed HTS significantly decreased mRNA expression of tight junction proteins occludin and ZO-1 but not claudin-1 in the jejunum. The number of intraepithelial lymphocytes and goblet cells were not significantly different (P > 0.05) between the groups. Collectively, these data suggest that dietary Trp supplementation at a certain level (0.75%) may negatively affect the small intestinal structure in weaned pig.

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Figures

Figure 1
Figure 1
mRNA abundance in the jejunum. a, bValues with different letters within the same row are different (P < 0.05).
See this image and copyright information in PMC

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