Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2016 May-Jun;32(3):575-9.
doi: 10.12669/pjms.323.9456.

Importance of immature platelet fraction as predictor of immune thrombocytopenic purpura

Arshi Naz  1 , Samina Naz Mukry  2 , Mahwish Rauf Shaikh  3 , Ali Raza Bukhari  4 , Tahir Sultan Shamsi  5
Affiliations

Importance of immature platelet fraction as predictor of immune thrombocytopenic purpura

Arshi Naz et al. Pak J Med Sci. 2016 May-Jun.

Abstract

Background and objective: Immune thrombocytopenic purpura (ITP) is a clinical syndrome in which a decreased number of circulating platelets (thrombocytopenia) manifests as a bleeding tendency, easy bruising (purpura) or extravasation of blood from capillaries into skin and mucous membranes (petechiae). The diagnosis of ITP can be made clinically on the basis of symptoms, we need to see if ITP can be confirmed in patients by quantification of residual RNA containing immature platelets (megakaryocytic mass) or immature platelets fraction (IPF) using automated hematology analyzers (Sysmex XE-2100).

Methods: In order to check the efficacy of IPF% parameter of Sysmex XE-2100 a total of 231 patients of thrombocytopenia were included in this study. Complete blood count (CBC) was estimated. The data was statistically analyzed by SPSS version 17.

Results: About 62 patients were diagnosed as ITP and 169 patients were diagnosed as non ITP on the basis of clinical history. The mean IPF % value of ITP patients was 16.39% and the IPF % value of Non ITP patients was ~7.69% respectively. There was no significant difference in IPF% values with respect to time between sampling and acquisition of complete blood count. The diagnostic sensitivity of IPF% as biomarker for ITP and non-ITP was 85.71% (95%CI: 84.04% to 85.96%) and 41.76% (95% CI: 39.87% to 43.65%).

Conclusion: The mean IPF % value by Sysmex XE-2100 can be used to predict ITP.

Keywords: Immune thrombocytopenic purpura (ITP); immature platelet fraction (IPF).

PubMed Disclaimer

Figures

Fig.1
Fig.1
Optical platelet scattergrams from a healthy individual with a normal IPF (a) and a patient with a high IPF (b). Mature platelets appear as blue dots, green dots represent the IPF with increased cell volume and higher fluorescence intensity compared to mature platelets.
See this image and copyright information in PMC

References

    1. George JN, Woolf SH, Raskob GE, Wasser JS, Aledort LM, Ballem PJ, et al. Idiopathic Thrombocytopenic Purpura:A Practice Guideline Developed by Explicit Methods for The American Society of Hematology. Blood. 1996;88(1):3–40. - PubMed
    1. Terrell DR, Beebe LA, Vesely SK, Neas BR, Segal JB, George JN. The incidence of immune thrombocytopenic purpura in children and adults:A critical review of published reports. Am J Hematol. 2010;85(3):174–180. - PubMed
    1. Ault KA, Rinder HM, Mitchell JG, Carmody MB, Vary CPH, Hillman RS. The significance of platelets with increased RNA content (reticulated platelets):a measure of the rate of thrombopoiesis. Am J Clin Pathol. 1992;98:637–646. - PubMed
    1. Briggs C, Kunka S, Hart D, Oguni S, Machin SJ. Assessment of an immature platelet fraction (IPF) in peripheral thrombocytopenia. Br J. Haematol. 2004;126:93–99. - PubMed
    1. Abe Y, Wada H, Tomatsu H, Sakaguchi A, Nishioka J, Yabu Y, et al. A simple technique to determine thrombopoiesis level using immature platelet fraction (IPF) Thromb Res. 2006;118:463–469. - PubMed

LinkOut - more resources