Regulation and role in pathogenicity of Erwinia chrysanthemi 3937 pectin methylesterase
- PMID: 2738029
- PMCID: PMC210169
- DOI: 10.1128/jb.171.7.4085-4087.1989
Regulation and role in pathogenicity of Erwinia chrysanthemi 3937 pectin methylesterase
Abstract
The gene pem, encoding the pectin methylesterase (PME) of Erwinia chrysanthemi 3937, was cloned and mutagenized by mini-Mu transposable elements. A second gene, pecY, which could act as a negative regulator of PME was found 5' to the pem gene. A PME-E. chrysanthemi derivative inoculate onto Saintpaulia plants was shown to be clearly noninvasive, demonstrating the important role of this enzyme in soft rot disease.
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