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. 2016 May-Jun;24(3):271-7.
doi: 10.1590/1678-775720150540.

Effects of budlein A on human neutrophils and lymphocytes

Carollinie Dias Knob  1 , Milena Silva  1 , Thaís Helena Gasparoto  1 , Carine Ervolino Oliveira  1 , Nádia Ghinelli Amôr  1 , Nilton Syogo Arakawa  2 , Fernando Batista Costa  3 , Ana Paula Campanelli  1
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Effects of budlein A on human neutrophils and lymphocytes

Carollinie Dias Knob et al. J Appl Oral Sci. 2016 May-Jun.

Abstract

Objective: In this study, we evaluated whether budlein A modulates the activation of innate and adaptive immune cells such as neutrophils and lymphocytes.

Material and methods: Our research group has investigated several plant species and several compounds have been isolated, identified, and their medical potential evaluated. Budlein A is a SL isolated from the species Aldama buddlejiformis and A. robusta (Asteraceae) and shows anti-inflammatory and anti-nociceptive activities. Advances in understanding how plant-derived substances modulate the activation of innate and adaptive immune cells have led to the development of new therapies for human diseases.

Results: Budlein A inhibited MPO activity, IL-6, CXCL8, IL-10, and IL-12 production and induces neutrophil apoptosis. In contrast, budlein A inhibited lymphocyte proliferation and IL-2, IL-10, TGF-β, and IFN-γ production, but it did not lead to cell death.

Conclusions: Collectively, our results indicate that budlein A shows distinct immunomodulatory effects on immune cells.

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Figures

Figure 1
Figure 1. Budlein A negatively regulated the neutrophil activation to fMLP and LPS. Neutrophils from healthy controls were cultured with medium only or budlein A (1 μM, 10 μM, or 100 μM), dexamethasone (100μM), budlein plus fMLP, or budlein plus LPS. (A) Analysis of myeloperoxidase activity (MPO). B) IL-6, CXCL8, IL-10, and IL-12 production were analyzed by ELISA. (C) Flow cytometric analysis of apoptosis. The bars represent the mean±SEM for each volunteer individually tested. * p<0.05
Figure 2
Figure 2. Budlein A inhibited T cell proliferation. (A) Flow cytometric analysis of proliferative response of PBMC cultured with Budlein A, dexamethasone, and PHA (1.5 mM) for 96 h. (B) IL-2, IFN-gamma, TGF-beta, and IL10 concentration in supernatant were analyzed by ELISA. (C) Flow cytometric analysis of apoptosis. The bars represent the mean±SEM of values obtained for each subject individually tested. *p<0.05, **p<0.01, ***p<0.001 was considered statistically significant when compared with the medium
Figure 3
Figure 3. Graphical abstract showing effects of Budlein A on human neutrophils and lymphocytes
See this image and copyright information in PMC

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