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. 2016 May-Jun;24(3):278-90.
doi: 10.1590/1678-775720150481.

TNF-alpha expression, evaluation of collagen, and TUNEL of Matricaria recutita L. extract and triamcinolone on oral ulcer in diabetic rats

Bruna Vasconcelos Oliveira  1 , Paulo Goberlânio Barros Silva  1 , Jacqueline de Santiago Nojosa  2 , Luiz André Cavalcante Brizeno  3 , Jamile Magalhães Ferreira  4 , Fabrício Bitú Sousa  1 , Mário Rogério Lima Mota  1 , Ana Paula Negreiros Nunes Alves  1
Affiliations

TNF-alpha expression, evaluation of collagen, and TUNEL of Matricaria recutita L. extract and triamcinolone on oral ulcer in diabetic rats

Bruna Vasconcelos Oliveira et al. J Appl Oral Sci. 2016 May-Jun.

Abstract

Objective: to evaluate the influence of Tumor Necrosis Factor alpha (TNF-α) and apoptosis in rats with DM treated with chamomile extract or triamcinolone.

Material and methods: Wistar male rats (210.0±4.2 g) were divided into five groups: negative control group (NCG) without diabetes; positive control group (PCG) with DM (alloxan, 45 mg/kg); and groups treated with chamomile extract (normoglycemic= NCG group and diabetic= DCG group) and with triamcinolone (TG). Traumatic ulcers were performed on all animals that received topical triamcinolone, chamomile extract or saline 12/12 hours for ten days.

Results: On days five and ten the animals were euthanized and the ulcers were analyzed by light microscopy, TUNEL assay, and immunohistochemically (TNF-α). The NCG (p=0.0062), PCG (p=0.0285), NCG (p=0.0041), and DCG (p<0.0001) groups were completely healed on the 10th day, however, there was no healing on the TG (p=0.5127) group. The TNF-α expression showed a significant reduction from the 5th to the 10th day in NCG (p=0.0266) and DCG (p=0.0062). In connective tissue, the TUNEL assay showed a significant reduction in the number of positive cells in NCG (p=0.0273) and CNG (p=0.0469) and in the epithelium only in CDG (p=0.0320).

Conclusions: Chamomile extract can optimize the healing of traumatic oral ulcers in diabetic rats through the reduction of apoptosis in the epithelium and TNF-α expression.

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Conflict of interest statement

AND SOURCE OF FUNDING The authors declare no sources of funding and conflicts of interest.

Figures

Figure 1
Figure 1. Microscopic analysis of induced oral ulcers (histological scores)
Figure 2
Figure 2. Macroscopic ulcers in the buccal mucosa in the Saline Treatment groups [Negative Control=Normoglycemic Rats (A and B); Positive Control Group=Diabetic Rats (C and D)], Chamomile Treatment Groups [Normoglycemic (E and F) and Diabetic Rats (G and H)], and Triamcinolone Group (I and J), respectively
Figure 3
Figure 3. Photomicrograph of ulceration in the buccal mucosa in the Saline Treatment groups [Negative Control=Normoglycemic Rats (A and B); Positive Control Group=Diabetic Rats (C and D)], Chamomile Treatment Groups [Normoglycemic (E and F) and Diabetic Rats (G and H)], and Triamcinolone Group (I and J), respectively. (Hematoxylin & eosin, Capital letters=100x; Small letters=200x)
Figure 4
Figure 4. Photomicrograph of ulceration in the buccal mucosa with picrosirius staining in Saline Treatment groups [Negative Control=Normoglycemic Rats (A and B); Positive Control Group=Diabetic Rats (C and D)], Chamomile Treatment Groups [Normoglycemic (E and F) and Diabetic Rats (G and H)], and Triamcinolone Group (I and J), respectively (Picrosirius, 200x)
Figure 5
Figure 5. Photomicrograph of ulceration in the buccal mucosa with TNF-α staining Saline Treatment groups [Negative Control=Normoglycemic Rats (A and B); Positive Control Group=Diabetic Rats (C and D)], Chamomile Treatment Groups [Normoglycemic (E and F) and Diabetic Rats (G and H)], and Triamcinolone Group (I and J), respectively (Immunohistochemistry, 400x)
Figure 6
Figure 6. Photomicrograph of ulceration in the buccal mucosa with TUNEL assay in Saline Treatment groups [Negative Control=Normoglycemic Rats (A and B); Positive Control Group=Diabetic Rats (C and D)], Chamomile Treatment Groups [Normoglycemic (E and F) and Diabetic Rats (G and H)], and Triamcinolone Group (I and J), respectively (TUNEL assay, 400x)
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