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. 2016 Oct;55(10):571-9.
doi: 10.1080/14992027.2016.1192693. Epub 2016 Jul 11.

A polymorphism in human estrogen-related receptor beta (ESRRβ) predicts audiometric temporary threshold shift

Ishan Bhatt  1 , Susan Phillips  2 , Scott Richter  3 , Denise Tucker  2 , Kristine Lundgren  2 , Robin Morehouse  4 , Vincent Henrich  5
Affiliations

A polymorphism in human estrogen-related receptor beta (ESRRβ) predicts audiometric temporary threshold shift

Ishan Bhatt et al. Int J Audiol. 2016 Oct.

Abstract

Objective: A non-synonymous single nucleotide polymorphism (rs61742642; C to T, P386S) in the ligand-binding domain of human estrogen-related receptor beta (ESRRβ) showed possible association to noise-induced hearing loss (NIHL) in our previous study.

Design: This study was conducted to examine the effect of the ESRRβ rs61742642 T variant on temporary threshold shift (TTS). TTS was induced by 10 minutes of exposure to audiometric narrow-band noise centered at 2000 Hz. Hearing thresholds and distortion product otoacoustic emissions input output function (DP IO) at 2000, 3000, and 4000 Hz were measured before and after the noise exposure.

Study sample: Nineteen participants with rs61742642 CT genotype and 40 participants with rs61742642 CC genotype were recruited for the study.

Results: Participants with the CT genotype acquired a significantly greater TTS without convincing evidence of greater DP IO temporary level shift (DPTLS) compared to participants with the CC genotype.

Conclusion: The results indicated that the ESRRβ polymorphism is associated with TTS. Future studies were recommended to explore molecular pathways leading to increased susceptibility to NIHL.

Keywords: Estrogen-Related Receptor Beta (ESRRβ); Noise-induced hearing loss (NIHL); Otoacoustic Emissions (OAE); Single Nucleotide Polymorphism (SNP); Temporary Threshold Shift (TTS).

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Conflict of interest statement

Declaration of interest: The authors declare no conflicts of interest.

Figures

Figure 1.
Figure 1.
Experimental paradigm: (A) Test sequence 1: Post-exposure behavioral audiometry was followed by DP IO function test for 30 participants (NCC=20 and NCT=10), (B) Test sequence 2: Post-exposure DP IO function test was followed by behavioral audiometry for 29 participants (NCC=20, NCT=9).
Figure 2.
Figure 2.
Pre-exposure audiometric thresholds: Mean thresholds at 1000–8000Hz for individuals carrying ESRRβ rs61742642CC variant (solid line) vs. CT variant (dashed line). Error bars indicate 95% confidence interval.
Figure 3.
Figure 3.
Pre-exposure DP area (left ear): DP area at 2000–6000Hz for individuals carrying ESRRβ rs61742642CC (solid line) vs. CT genotype (dashed line). Error bars indicate 95% confidence interval.
Figure 4.
Figure 4.
Pre-exposure TEOAE one-third octave SNR (left ear): SNR values at 10004000Hz for individuals carrying ESRRβ rs61742642CC (solid line) vs. CT genotype (dashed line). Error bars indicate 95% confidence interval.
Figure 5.
Figure 5.
Post-exposure findings (left ear): Frequency-specific TTS values for individuals carrying ESRRβ rs61742642CC (blank bar) vs. CT genotype (gray bar). Error bars indicate 95% confidence interval.
Figure 6.
Figure 6.
Post-exposure findings (left ear): Individual values of TTS vs. DPTLS for musicians carrying ESRRβ rs61742642CC genotype (circle) vs. CT genotype (cross). Highlighted data points show results of the biological brothers. Musician brother with the ESRRβ CT genotype and four years of professional music exposure history acquired more TTS compared to his brother with ESRRβ CC genotype and 12 years of music exposure history.
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References

    1. American National Standards Institute (ANSI). 1999. Maximum permissible ambient noise level for audiometric test rooms. ANSI, S3.1–1999.
    1. Ben Saïd M, Ayedi L, Mnejja M, Hakim B, Khalfallah A et al. 2011. A novel missense mutation in the ESRRΒ gene causes DFNB35 hearing loss in a Tunisian family. Eur J Med Genet, 54(6), 535–541. - PubMed
    1. Brozkova DS, Lastuvkova J, Machalova E, Lisonova J, Trkova M et al. 2012. DFNB35 due to a novel mutation in the ESRRΒ gene in a Czech consanguineous family. Int J Ped Otorhinolaryngol, 76, 1681–1684. - PubMed
    1. Chen J & Nathans J 2007. Estrogen-related receptor beta/NR3B2 controls epithelial cell fate and endolymph production by the Stria Vascularis. Develop Cell, 13(3), 325–337. - PubMed
    1. Collet L 1993. Use of otoacoustic emissions to explore the medial olivocochlear system in humans. Br J Audiol, 27(2), 155–159. - PubMed

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