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. 2016 Sep;57(5):1282-5.
doi: 10.3349/ymj.2016.57.5.1282.

Platelet-Rich Fibrin Lysate Can Ameliorate Dysfunction of Chronically UVA-Irradiated Human Dermal Fibroblasts

Yohanes Widodo Wirohadidjojo  1 , Arief Budiyanto  2 , Hardyanto Soebono  2
Affiliations

Platelet-Rich Fibrin Lysate Can Ameliorate Dysfunction of Chronically UVA-Irradiated Human Dermal Fibroblasts

Yohanes Widodo Wirohadidjojo et al. Yonsei Med J. 2016 Sep.

Abstract

To determine whether platelet-rich fibrin lysate (PRF-L) could restore the function of chronically ultraviolet-A (UVA)-irradiated human dermal fibroblasts (HDFs), we isolated and sub-cultured HDFs from six different human foreskins. HDFs were divided into two groups: those that received chronic UVA irradiation (total dosages of 10 J cm⁻²) and those that were not irradiated. We compared the proliferation rates, collagen deposition, and migration rates between the groups and between chronically UVA-irradiated HDFs in control and PRF-L-treated media. Our experiment showed that chronic UVA irradiation significantly decreased (p<0.05) the proliferation rates, migration rates, and collagen deposition of HDFs, compared to controls. Compared to control media, chronically UVA-irradiated HDFs in 50% PRF-L had significantly increased proliferation rates, migration rates, and collagen deposition (p<0.05), and the migration rates and collagen deposition of chronically UVA-irradiated HDFs in 50% PRF-L were equal to those of normal fibroblasts. Based on this experiment, we concluded that PRF-L is a good candidate material for treating UVA-induced photoaging of skin, although the best method for its clinical application remains to be determined.

Keywords: Collagen; cell migration; cell proliferation; platelet-rich fibrin; ultraviolet-A.

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Conflict of interest statement

The authors have no financial conflicts of interest.

Figures

Fig. 1
Fig. 1. Migration of normal human dermal fibroblasts in Scratch-Assay. (A) Immediately after scratching. (B) 24 hours later. (C) 48 hours later. (D) 72 hours later.
Fig. 2
Fig. 2. The effect of PRF-L on proliferation index, collagen deposition, and migration rates of UVA-irradiated HDFs. *p<0.05, †p<0.01. PRF-L, platelet-rich fibrin lysate; UVA, ultraviolet-A; HDFs, human dermal fibroblasts.
See this image and copyright information in PMC

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