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. 2016 May;19(5):573-8.
doi: 10.22038/IJBMS.2016.6943.

Breviscapine prevents downregulation of renal water and sodium transport proteins in response to unilateral ureteral obstruction

Yang Mei  1 , Zhuang Yangyang  1 , Luo Shuai  2 , Jin Hao  2 , Yang Yirong  2 , Cai Yong  2 , Xia Peng  2 , Chen Bicheng  3 , Zhang Yan  2
Affiliations

Breviscapine prevents downregulation of renal water and sodium transport proteins in response to unilateral ureteral obstruction

Yang Mei et al. Iran J Basic Med Sci. 2016 May.

Erratum in

Abstract

Objectives: Our recent report indicates that breviscapine play a protective role of the kidney by down-regulating transforming growth factor-β1(TGF-β1), α-smooth muscle actin (α-SMA) and alleviating interstitial fibrosis following unilateral ureteral obstruction (UUO). In this study, we investigate the effect of breviscapine on changes of renal water and sodium transport proteins in response to UUO.

Materials and methods: Male Sprague-Dawley rats were divided into 3 groups, sham group, UUO group and UUO treat with breviscapine. After 4, 7 and 14 days, histologic changes and interstitial collagen were determined microscopically following hematoxylin and eosin (H&E) and Masson's trichrome staining. The expression of Aquaporins (AQP-2) and γ-epithelial sodium channel (γ-ENaC) were investigated using immunohistochemistry and Western blot in each group.

Results: Breviscapine treatment decrease the tubular injury index and the degree of interstitial collagen deposition significantly compared with the UUO group (P<0.05). Breviscapine treatment also significantly reduced downregulation of AQP2 and γ-ENaC compared to those subjected to the same time course of obstruction in UUO group (P<0.05).

Conclusion: These results demonstrate that breviscapine could prevent downregulation of renal water and sodium transport proteins in response to UUO so as to protect obstructed kidney.

Keywords: Breviscapine; Kidney; Ureteral obstruction; Water and sodium trans-; port proteins.

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Figures

Figure 1
Figure 1
a: Breviscapine attenuated the histological changes in the obstructed kidney induced by UUO. Representative hematoxylin–eosin staining micrographs of (A, B, C) sham group, (D, E, F) UUO group and (G, H, I) breviscapine group. b: Breviscapine attenuated the interstitial collagen deposition in the obstructed kidney induced by UUO. Representative Masson’s trichrome staining micrographs of (A, B, C) sham group, (D, E, F) UUO group and (G, H, I) breviscapine group. Original magnification×200. ※P<0.05 in comparison with Sham group. ※P<0.05 in comparison with UUO group
Figure 2
Figure 2
a: Breviscapine suppressed the downregulation of AQP2 in the obstructed kidney induced by UUO. Representative micrographs of (A, B, C) sham group, (D, E, F) UUO group and (G, H, I) Breviscapine group. b: Breviscapine suppressed the downregulation of γ-ENaC in the obstructed kidney induced by UUO. Representative Masson’s trichrome staining micrographs of (A, B, C) sham group, (D, E, F) UUO group and (G, H, I) breviscapine group. Original magnification×200. ※P<0.05 in comparison with Sham group at the same time point. ※P<0.05 in comparison with UUO group at the same time point
Figure 3
Figure 3
A. Breviscapine suppressed the down-regulation of AQP2 in the obstructed kidney assessed by Western blot assay. B. Breviscapine suppressed the down-regulation of γ-ENaC in the obstructed kidney assessed by Western blot assay. ※P<0.05 in comparison with Sham group. ※P<0.05 in comparison with UUO group
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