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. 2016 May;53(5):2270-8.
doi: 10.1007/s13197-016-2185-0. Epub 2016 May 27.

Culture media optimization of Porphyridium purpureum: production potential of biomass, total lipids, arachidonic and eicosapentaenoic acid

Mysore Doddaiah Kavitha  1 , Shanmugam Kathiresan  2 , Sila Bhattacharya  3 , Ravi Sarada  1
Affiliations

Culture media optimization of Porphyridium purpureum: production potential of biomass, total lipids, arachidonic and eicosapentaenoic acid

Mysore Doddaiah Kavitha et al. J Food Sci Technol. 2016 May.

Abstract

Porphyridium purpureum a red marine microalga is known for phycobiliproteins (PB), polyunsaturated fatty acids and sulphated exopolysaccharides. In the present study, effects of media constituents for the production of different polyunsaturated fatty acids from P. purpureum were considered using a response surface methodology (RSM). A second order polynomial was used to predict the response functions in terms of the independent variables such as the concentrations of sodium chloride, magnesium sulphate, sodium nitrate and potassium dihydrogen phosphate. The response functions were production of biomass yield, total lipid and polyunsaturated fatty acids like arachidonic acid (AA 20:4) and eicosapentaenoic acid (EPA 20:5). Results corroborated that maximum Biomass (0.95 gL(-1)) yield was at the concentrations of sodium chloride (14.89 gL(-1)), magnesium sulfate (3.93 gL(-1)) and sodium nitrate (0.96 gL(-1)) and potassium dihydrogen phosphate (0.09 gL(-1)). Optimum total lipid (17.9 % w/w) and EPA (34.6 % w/w) content was at the concentrations of sodium chloride (29.98 gL(-1)), magnesium sulfate (9.34 gL(-1)) and sodium nitrate (1.86 gL(-1)). Variation in concentration of potassium dihydrogen phosphate for both lipid (0.01gL(-1)) and EPA content (0.20 gL(-1)) was observed. The optimum conditions for biomass, total lipid, AA and EPA varied indicating their batch mode of growth and interaction effect of the salt.

Keywords: Eicosapentaenoic acid; Fatty acids; Optimization; Porphyridium; Response surface methodology (RSM).

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Figures

Fig. 1
Fig. 1
Response surface plot for (a) biomass production and (b) AA production when magnesium sulfate and potassium dihydrogen phosphate concentration kept constant
Fig. 2
Fig. 2
GCMS profile of FAME of P. purpureum biomass indicating presence of AA
Fig. 3
Fig. 3
Response surface plot for (a) EPA when phosphate and nitrate concentration kept constant and (b) total lipid production when sulfate and potassium dihydrogen phosphate concentration kept constant
See this image and copyright information in PMC

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