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Comment
. 2016 Jun;5(3):338-42.
doi: 10.21037/tlcr.2016.05.01.

Digital detection of T790M-yes or no to an ultrasensitive assay

Wei Wang  1 , Elena Karampini  1 , Lúcia L Correia  1 , Frank M McCaughan  1
Affiliations
Comment

Digital detection of T790M-yes or no to an ultrasensitive assay

Wei Wang et al. Transl Lung Cancer Res. 2016 Jun.
No abstract available

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Conflict of interest statement

Conflicts of Interest: The authors have no conflicts of interest to declare.

Figures

Figure 1
Figure 1
Simple schematic of digital PCR. Sample DNA is diluted, to on average less than a haploid genome per microdroplet prior to the PCR. Each microdroplet contains the reagents required for the PCR reaction and the mutant and wild type probes which are labelled with different fluorophores. The PCR reaction is performed in the standard way and each droplet then tested using laser excitation for the presence or absence of the target DNA locus. In this case the rare variant T790M is represented in red and is present in only one microdroplet, while the wild type allele is present in the majority of microdroplets. A simple (and software automated) mathematical transformation is performed to estimate the mutant allele frequency (MAF) of T790M. EGFR, epidermal growth factor receptor.
See this image and copyright information in PMC

Comment on

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