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. 2016:2016:7694921.
doi: 10.1155/2016/7694921. Epub 2016 Jun 20.

Effect of Toll-Like Receptor 4 on Synovial Injury of Temporomandibular Joint in Rats Caused by Occlusal Interference

Jingjing Kong  1 , Yingying Yang  2 , Shuzhen Sun  1 , Jianli Xie  1 , Xuefen Lin  3 , Ping Ji  3
Affiliations

Effect of Toll-Like Receptor 4 on Synovial Injury of Temporomandibular Joint in Rats Caused by Occlusal Interference

Jingjing Kong et al. Mediators Inflamm. 2016.

Abstract

Synovitis is an important disease that causes intractable pain in TMJ. Some investigations suggested that the increasing expression of IL-1β secreted by synovial lining cells plays an important role in synovial inflammation and cartilage destruction in TMJ. In our previous research, the results demonstrated that TLR4 is involved in the expression of IL-1β in SFs from TMJ with lipopolysaccharide stimulation. However, the inflammatory response that occurred in synovial membrane is not caused by bacterial infection. In the current study, we investigated whether or not TLR4 participates in the inflammatory responses and the expression of IL-1β in synovial membrane of rats induced by occlusal interference. The results showed that obvious inflammation changes were observed in the synovial membranes and the expression of TLR4 and IL-1β was increased at both mRNA and protein levels in the occlusal interference rats. In addition, the inflammation reactions and the increased expression of IL-1β could be restrained by treatment with TAK-242, a blocker of TLR4 signaling. The results prompted us that the activation of TLR4 may be involved in the inflammatory reactions and increased expression of IL-1β in patients with synovitis and participate in the mechanisms of the initiation and development of synovial injury by regulating the expression of inflammatory mediators like IL-1β in synovial membranes.

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Figures

Figure 1
Figure 1
Histological examination of synovial membranes. (a) Control group. (b) Occlusal interference group. (c) TAK-242 group. (d) The histopathological score of each group. As the results shown, in comparison with the control group, the histopathological score was significantly increased in the occlusal interference group. However, this effect could be inhibited significantly after treatment with the TAK-242. Data shows all the values from independent samples of n = 6, ∗ P < 0.05 versus control group and # P < 0.05 versus occlusal interference group.
Figure 2
Figure 2
The expression of TLR4 in the synovial membranes. (a) Immunohistochemical staining for TLR4 in the membranes of the control group. (b) Immunohistochemical staining for TLR4 in the membranes of occlusal interference group. (c) Immunohistochemical staining for TLR4 in the membranes of TAK-242 group. (d) The mean optical density of each group. (e) The relative mRNA expression of TLR4 of each group. As the results shown, in comparison with the control group, the expression of TLR4 was significantly increased in the occlusal interference group at both protein and mRNA levels. However, this effect could be inhibited significantly after treatment with the TAK-242. Data shows all the values from independent samples of n = 6, ∗ P < 0.05 versus control group and # P < 0.05 versus occlusal interference group.
Figure 3
Figure 3
Effect of TLR4 on the expression of IL-1β in the synovial membranes. (a) Immunohistochemical staining for IL-1β in the membranes of the control group. (b) Immunohistochemical staining for IL-1β in the membranes of occlusal interference group. (c) Immunohistochemical staining for IL-1β in the membranes of TAK-242 group. (d) The mean optical density of each group. (e) The relative mRNA expression of IL-1β of each group. As the results shown, in comparison with the control group, the expression of IL-1β was significantly increased in the occlusal interference group at both protein and mRNA levels. However, this effect could be inhibited significantly after treatment with the TAK-242. Data shows all the values from independent samples of n = 6, ∗ P < 0.05 versus control group and # P < 0.05 versus occlusal interference group.
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