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. 1989 Feb 1;176(2):365-7.
doi: 10.1016/0003-2697(89)90324-2.

Modified method for peptide mapping of collagen chains using cyanogen bromide-cleavage of protein within polyacrylamide gels

B P Sokolov  1 , B M Sher, V N Kalinin
Affiliations

Modified method for peptide mapping of collagen chains using cyanogen bromide-cleavage of protein within polyacrylamide gels

B P Sokolov et al. Anal Biochem. .

Abstract

A highly efficient method for cyanogen bromide (CNBr)-mapping of collagen peptides is described. This method was developed based on polypeptide cleavage by CNBr within gel slices according to Barsh et al. [1981) Collagen Relat. Res. 1, 543-548). The proposed method has the following advantages: (i) Analysis of both radiolabeled and unlabeled collagens is possible; (ii) CNBr-cleavage of polypeptides is performed in gel pieces which contain individual bands; (iii) The peptide losses are minimized, offering a more complete analysis of collagens including the low molecular weight CNBr-peptides.

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