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. 2014 Oct 28:2014:676013.
doi: 10.1155/2014/676013. eCollection 2014.

Effects of Atrazine on Reproductive Health of Nondiabetic and Diabetic Male Rats

Dinesh Babu Jestadi  1 , Alugoju Phaniendra  1 , Undru Babji  1 , Bhavatharini Shanmuganathan  1 , Latha Periyasamy  1
Affiliations

Effects of Atrazine on Reproductive Health of Nondiabetic and Diabetic Male Rats

Dinesh Babu Jestadi et al. Int Sch Res Notices. .

Abstract

The aim of the present study was to investigate the effects of low dose of atrazine on reproductive system of male Wistar rats. 16 rats were divided into four groups of four animals each. Group I (nondiabetic) and group III (diabetic) animals served as controls that received safflower oil (300 μL/kg bw/day), respectively. Group II (nondiabetic) and group IV (diabetic) animals received atrazine (300 μg/kg bw/day). Nonsignificant decrease in the activities of antioxidant and steroidogenic enzymes and sperm parameters suggests that atrazine did not produce any effect on reproductive system of rats. Histological findings also revealed that atrazine at a dose of 300 μg/kg bw did not produce any testicular toxic effects in nondiabetic and diabetic atrazine treated rats. Low dose of atrazine did not show reproductive toxicity in rats. To know the effects of atrazine in diabetic rats further studies have to be carried out with increased concentration of atrazine.

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Figures

Figure 1
Figure 1
Effects of atrazine on lipid peroxidation. The data are represented as mean ± SD (n = 4) and evaluated by one-way analysis of variance (ANOVA) confirmed that the groups are not significantly differed (P > 0.05). NC = nondiabetic control rats, NA = nondiabetic atrazine treated rats, DC = diabetic control rats, and DA = diabetic atrazine treated rats.
Figure 2
Figure 2
Effects of atrazine on H2O2 generation. The data are represented as mean ± SD (n = 4) and evaluated by one-way analysis of variance (ANOVA) confirmed that the groups are not significantly differed (P > 0.05). NC = nondiabetic control rats, NA = nondiabetic atrazine treated rats, DC = diabetic control rats, and DA = diabetic atrazine treated rats.
Figure 3
Figure 3
Effects of atrazine on 3β-HSD. The data are represented as mean ± SD (n = 4) and evaluated by one-way analysis of variance (ANOVA) confirmed that the groups are not significantly differed (P > 0.05). NC = nondiabetic control rats, NA = nondiabetic atrazine treated rats, DC = diabetic control rats, and DA = diabetic atrazine treated rats.
Figure 4
Figure 4
Effects of atrazine on 17β-HSD. The data are represented as mean ± SD (n = 4) and evaluated by one-way analysis of variance (ANOVA) confirmed that the groups are not significantly differed (P > 0.05). NC = nondiabetic control rats, NA = nondiabetic atrazine treated rats, DC = diabetic control rats, and DA = diabetic atrazine treated rats.
Figure 5
Figure 5
Light microscopic images of the section of testis. (a), (b), (c), and (d) are hematoxylin and eosin (H&E 40x) stained section of testis of nondiabetic control (NC), nondiabetic atrazine treated rats (NA), diabetic control rats (DC), and diabetic atrazine treated rats (DA), respectively. (a) and (b) show normal spermatogonia, Sertoli cells, and Leydig cells with accumulation of spermatozoa within the seminiferous tubules. (c) and (d) show reduced numbers of spermatogonia, Sertoli cells, and Leydig cells with drastic reduction of spermatozoa within the seminiferous tubules.
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