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. 2016 Aug;22(8):1474-6.
doi: 10.3201/eid2208.151917.

Whole-Genome Sequencing Detection of Ongoing Listeria Contamination at a Restaurant, Rhode Island, USA, 2014

Jonathan S Barkley, Michael Gosciminski, Adam Miller

Whole-Genome Sequencing Detection of Ongoing Listeria Contamination at a Restaurant, Rhode Island, USA, 2014

Jonathan S Barkley et al. Emerg Infect Dis. 2016 Aug.

Abstract

In November 2014, the Rhode Island Department of Health investigated a cluster of 3 listeriosis cases. Using whole-genome sequencing to support epidemiologic, laboratory, and environmental investigations, the department identified 1 restaurant as the likely source of the outbreak and also linked the establishment to a listeriosis case that occurred in 2013.

Keywords: PFGE; WGS; bacteria; bacterial pathogen; contamination source; environmental contamination; environmental investigation; epidemiologic investigation; food processing; food service establishments; foodborne infections; listeria; listeriosis; outbreak; pulsed-field gel electrophoresis; whole-genome sequencing.

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Figures

Figure
Figure
Median (minimum–maximum) allele differences and pulsed-field gel electrophoresis (PFGE ) patterns for Listeria monocytogenes isolates from clinical and food samples associated with a 2014 cluster of listeriosis cases and a 2013 listeriosis case, Rhode Island, USA. Allele differences were determined by whole-genome multilocus sequence typing (wgMLST). Adapted from data provided by the Enteric Diseases Laboratory Branch, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention (Atlanta, GA, USA).
See this image and copyright information in PMC

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