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. 2016 Feb 19;2(2):e00076.
doi: 10.1016/j.heliyon.2016.e00076. eCollection 2016 Feb.

Partial structural characterization of pectin cell wall from Argania spinosa leaves

Kadda Hachem  1 , Yasmina Benabdesslem  2 , Samia Ghomari  3 , Okkacha Hasnaoui  2 , Meriem Kaid-Harche  4
Affiliations

Partial structural characterization of pectin cell wall from Argania spinosa leaves

Kadda Hachem et al. Heliyon. .

Abstract

The pectin polysaccharides from leaves of Argania spinosa (L.) Skeels, collected from Stidia area in the west coast of northern Algeria, were investigated by using sequential extractions and the resulting fractions were analysed for monosaccharide composition and chemical structure. Water-soluble pectic (ALS-WSP) and chelating-soluble pectic (ALS-CSP) fractions were obtained, de-esterified and fractionated by anion-exchange chromatography and characterized by sugar analysis combined with methylation analysis and (1)H and (13)C NMR spectroscopy. The data reveal the presence of altering homogalacturonan (HG) and rhamnogalacturonan I (RG-I) in both pectin fraction. The rhamnogalacturonan I (RG-I) are consisted of a disaccharide repeating unit [→ α-D-GalpA-1,2-α-L-Rhap-1,4 →] backbone, with side chains contained highly branched α-(1 → 5)-linked arabinan and short linear β-(1 → 4)-linked galactan, attached to O-4 of the rhamnosyl residues.

Keywords: Applied sciences; Biochemistry; Biological sciences; Natural product chemistry; Plant biology.

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Figures

Fig. 1
Fig. 1
Ion-exchange chromatography fractionation of (ALS-WSP) and (ALS-WSP).
Fig. 2
Fig. 2
1H NMR spectra (300.0 K, 400.13 MHz) of (a) ALS-WSP(3) and (b) ALS-WSP(3).
Fig. 3
Fig. 3
13C NMR spectra (300.0 K, 100.62 MHz) of (a) ALS-WSP(3) and (b) ALS-WSP(3).
Fig. 4
Fig. 4
Schematic structure of ALS-WSP(3) and ALS-WSP(3).
See this image and copyright information in PMC

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