Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2016 Nov;44(11):e1132-e1136.
doi: 10.1097/CCM.0000000000001851.

Neutrophil Fluorescence: A New Indicator of Cell Activation During Septic Shock-Induced Disseminated Intravascular Coagulation

Laure Stiel  1 , Xavier Delabranche, Anne-Cécile Galoisy, François Severac, Florence Toti, Laurent Mauvieux, Ferhat Meziani, Julie Boisramé-Helms
Affiliations

Neutrophil Fluorescence: A New Indicator of Cell Activation During Septic Shock-Induced Disseminated Intravascular Coagulation

Laure Stiel et al. Crit Care Med. 2016 Nov.

Abstract

Objective: To investigate the contribution of neutrophil activation as innate immune cells during septic shock-induced disseminated intravascular coagulation.

Design: Prospective study.

Setting: One University Hospital ICU.

Participants: Hundred patients with septic shock. Thirty-five patients had disseminated intravascular coagulation according to Japanese Association for Acute Medicine 2006 score.

Intervention: None.

Measurements and main results: Neutrophil chromatin decondensation was assessed by measuring neutrophil fluorescence (NEUT-side-fluorescence light) labeled by a fluorochrome-based polymethine reagent using a routine automated flow cytometer Sysmex XN20 (Sysmex, Kobe, Japan) and neutrophil-derived CD66b microparticles by prothrombinase assay. Measurements in disseminated intravascular coagulation and no disseminated intravascular coagulation patients showed that a mean value of NEUT-side-fluorescence light above 57.3 arbitrary units had a sensitivity of 90.91% and a specificity of 80.60% for disseminated intravascular coagulation diagnosis. NEUT-side-fluorescence light was correlated to the CD66b microparticles/neutrophil count, a surrogate of neutrophil activation associated with septic shock-induced disseminated intravascular coagulation.

Conclusion: NEUT-side-fluorescence light, routinely available, could prove an accurate biomarker of neutrophil activation.

PubMed Disclaimer