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. 2016 Oct:161:319-324.
doi: 10.1016/j.chemosphere.2016.07.014. Epub 2016 Jul 19.

Characterizing a novel and sensitive method to measure dsRNA in soil

Joshua R Fischer  1 , Fatima Zapata  2 , Samuel Dubelman  2 , Geoffrey M Mueller  2 , Peter D Jensen  2 , Steven L Levine  2
Affiliations

Characterizing a novel and sensitive method to measure dsRNA in soil

Joshua R Fischer et al. Chemosphere. 2016 Oct.

Abstract

Performing environmental assessments for double-stranded RNA-based agricultural products require the development of sensitive and selective methods to measure biodegradation rates of dsRNAs. We developed and characterized a novel analytical procedure that uses a molecular hybridization assay (QuantiGene(®)) to accurately measure dsRNA extracted from diverse soils. In this report, we utilize this method to demonstrate that two dsRNAs with distinct size, structure, and sequence degrade rapidly in soil with indistinguishable kinetics.

Keywords: DvSnf7; Environmental fate; QuantiGene; Soil; dsRNA.

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