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. 2016 Jul 22;60(8):5102-4.
doi: 10.1128/AAC.00785-16. Print 2016 Aug.

Mobilization of blaBKC-1 by ISKpn23?

Sally R Partridge  1
Affiliations

Mobilization of blaBKC-1 by ISKpn23?

Sally R Partridge. Antimicrob Agents Chemother. .
No abstract available

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Figures

FIG 1
FIG 1
(A) Diagram of the complete sequence of p60136 (GenBank accession no. KP689347) showing the proposed ISKpn23-blaBKC-1 TU, compared with aphA6 in the A. guillouiae chromosome (AP014630). “1” indicates position 1 in the p60136 sequence in KP689347, which was reversed to draw the diagram. ISKpn23 is shown as a box, with the pointed end indicating IRR. Different types of labeled arrows show the extents and directions of resistance and plasmid backbone genes. oriV regions are indicated by shaded boxes labeled “V.” The regions common to both sequences are indicated by shading, and the positions of the 5-bp DRs (TTACT) flanking the proposed TU in p60136 and the single copy of this sequence in A. guillouiae are shown. (B) Sequences around the insertion site in A. guillouiae (middle line) and flanking the proposed TU in p60136 (top and bottom lines). Differences are shown in lowercase letters in the A. guillouiae sequence. The proposed IRL of ISKpn23 is shown in bold typeface, the aphA6 start codon is bold and underlined, and the proposed DRs are underlined. (C) Potential ISKpn23-mediated TU in Enterobacter cloacae (JCKL01000012), carrying aac(3)-IIb inserted in a partial insertion sequence, IS4321 (gray), and in Pseudomonas aeruginosa (JTQL01000147) (15), carrying a putative aac(3) gene inserted in Tn5393. (D) Comparison of the ends of ISKpn23 in p60136, ISKpn23 in the NCBI WGS database (differences are underlined in each sequence), and IS1247 (as proposed in reference 8), with IRL and IRR in uppercase letters (16 bp, as listed in ISfinder). (E) Comparison of sequences upstream of the point of insertion of the proposed TU in p60136.
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Comment in

  • Reply to "Mobilization of blaBKC-1 by ISKpn23?".
    Nicoletti AG, Marcondes MF, Martins WM, Almeida LG, Nicolás MF, Vasconcelos AT, Oliveira V, Gales AC. Nicoletti AG, et al. Antimicrob Agents Chemother. 2016 Jul 22;60(8):5105. doi: 10.1128/AAC.01014-16. Print 2016 Aug. Antimicrob Agents Chemother. 2016. PMID: 27451438 Free PMC article. No abstract available.

References

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